Botulinum neurotoxin type F (F) is a 1278-residue protein from Clostridium botulinum (strain Langeland / NCTC 10281 / Type F). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: A7GBG3.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 87.3 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 56% |
| 70 to 90 | Confident: backbone generally right | 36% |
| 50 to 70 | Low: treat with caution | 7% |
| Below 50 | Very low: often disordered regions | 2% |
What pLDDT means and how to read it
Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure (PubMed:14423425). Precursor of botulinum neurotoxin F which may have 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them (PubMed:19476346, PubMed:19650874). Upon synaptic vesicle recycling the toxin is taken up…
Heterodimer; disulfide-linked heterodimer of a light chain (LC) and a heavy chain (HC). The LC has the proteolytic/pharmacological activity, while the N- and C-terminal of the HC mediate channel formation and toxin binding, respectively. Interacts with host synaptic vesicle glycoproteins SV2A, SV2B and SV2C (PubMed:19650874). HC interacts with a complex including at least host SV2 and…
Secreted, Host cytoplasm, host cytosol, Host synapse, host presynaptic cell membrane, Host cytoplasmic vesicle, host secretory vesicle, host synaptic vesicle membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3RSJ | X-ray | 2.0 Å | A/B/C/D=866-1278 |
| 3FIE | X-ray | 2.1 Å | A/B=1-419 |
| 3FUQ | X-ray | 2.1 Å | A=862-1278 |
| 3FII | X-ray | 2.17 Å | A=1-419 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.