DASH complex subunit ASK1 (ASK1) is a 508-residue protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: G0S8F9.
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The mean pLDDT of this model is 56.0 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 12% |
| 70 to 90 | Confident: backbone generally right | 9% |
| 50 to 70 | Low: treat with caution | 27% |
| Below 50 | Very low: often disordered regions | 53% |
What pLDDT means and how to read it
Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerization to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase (By similarity). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and microtubules (By similarity). Kinetochores also serve as an input point for the spindle…
Component of the DASH complex consisting of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with a stoichiometry of one copy of each subunit per complex (PubMed:29724956). Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and organizes the rod-like NDC80 complexes of the outer kinetochore (PubMed:29724956). On cytoplasmic microtubules, DASH…
Chromosome, centromere, kinetochore, Cytoplasm, cytoskeleton, spindle, Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 6CFZ | EM | 4.5 Å | A=12-88 |
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