Histone H3 (H3-3B) is a 92-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Jun 2022. UniProt accession: K7EMV3.
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The mean pLDDT of this model is 83.0 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 55% |
| 70 to 90 | Confident: backbone generally right | 8% |
| 50 to 70 | Low: treat with caution | 36% |
| Below 50 | Very low: often disordered regions | 1% |
What pLDDT means and how to read it
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5B78 | X-ray | 1.4 Å | B=2-26 |
| 5B77 | X-ray | 1.55 Å | B=2-26 |
| 5B76 | X-ray | 1.65 Å | B=2-26 |
| 5B75 | X-ray | 1.7 Å | B=2-26 |
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