Ubiquitin conjugation factor E4 B (UBE4B) is a 1302-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: O95155.
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The mean pLDDT of this model is 75.0 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 51% |
| 70 to 90 | Confident: backbone generally right | 22% |
| 50 to 70 | Low: treat with caution | 2% |
| Below 50 | Very low: often disordered regions | 25% |
What pLDDT means and how to read it
Acts as an E3 ubiquitin ligase and E4 ubiquitin chain elongation enzyme specifically involved in polyubiquitin chain assembly (PubMed:21317885). Plays an essential role in cardiac development and in the protection of neurons from degeneration evoked by ER stress (By similarity). Is recruited to early endosomes via interaction with the ESCRT-0 component HGS, where it ubiquitinates membrane protein cargo such as EGFR and APP to promote their sorting into multivesicular bodies and subsequent lysosomal degradation (PubMed:24344129, PubMed:32841720). Promotes MDM2-mediated polyubiquitination and subsequent degradation of the tumor suppressor TP53 (PubMed:21317885). May regulate myosin assembly…
Interacts with VCP/p97. Interacts with STUB1/CHIP and UNC45B. Interacts with HGS; this interaction recruits UBE4B to endosomal membranes in response to EGFR activation (PubMed:24344129). Interacts with MDM2 (PubMed:21317885). Interacts with ubiquitin-conjugating enzyme E2s UBCH5C AND UBC4 (PubMed:20696396)
Early endosome, Cytoplasm, Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5O75 | X-ray | 1.48 Å | A=1226-1302 |
| 3L1X | X-ray | 2.6 Å | A=1208-1302 |
| 3L1Z | X-ray | 3.17 Å | B=1208-1302 |
| 2KRE | NMR | A=1208-1302 |
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