Tumor necrosis factor (TNF) is a 233-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P01375.
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The mean pLDDT of this model is 84.6 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 63% |
| 70 to 90 | Confident: backbone generally right | 12% |
| 50 to 70 | Low: treat with caution | 18% |
| Below 50 | Very low: often disordered regions | 7% |
What pLDDT means and how to read it
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective…
Homotrimer. Interacts with SPPL2B
Cell membrane, Membrane, Secreted
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 9OJO | X-ray | 1.36 Å | A/B/C=77-233 |
| 5UUI | X-ray | 1.4 Å | A=77-233 |
| 4Y6O | X-ray | 1.6 Å | C/D=17-23 |
| 2E7A | X-ray | 1.8 Å | A/B/C=77-233 |
| 4TSV | X-ray | 1.8 Å | A=84-233 |
| 9OJS | X-ray | 1.85 Å | A/B/C=77-233 |
| 5M2J | X-ray | 1.9 Å | A=77-233 |
| 9BN7 | X-ray | 1.92 Å | A/B/C=77-233 |
| 2AZ5 | X-ray | 2.1 Å | A/B/C/D=86-233 |
| 3L9J | X-ray | 2.1 Å | T=85-233 |
| 7JRA | X-ray | 2.1 Å | A/B/C=77-233 |
| 5M2I | X-ray | 2.15 Å | A/B/C/D/E/F=77-233 |
| 7KP9 | X-ray | 2.15 Å | A/B/C=77-233 |
| 9OJY | X-ray | 2.16 Å | A/B/C/D/E/F=77-233 |
| 1A8M | X-ray | 2.3 Å | A/B/C=77-233 |
| 5M2M | X-ray | 2.3 Å | A/B/C/G/I/M=77-233 |
| 7KPA | X-ray | 2.3 Å | A/B/C=77-233 |
| 21TW | EM | 2.43 Å | B/C/D=77-233 |
| 2ZJC | X-ray | 2.5 Å | A/B/C=77-233 |
| 5TSW | X-ray | 2.5 Å | A/B/C/D/E/F=84-233 |
Showing 20 of 52 experimental structures (best resolution first).
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