Guanine nucleotide-binding protein G(o) subunit alpha (GNAO1) is a 354-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P09471.
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The mean pLDDT of this model is 94.5 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 87% |
| 70 to 90 | Confident: backbone generally right | 11% |
| 50 to 70 | Low: treat with caution | 1% |
| Below 50 | Very low: often disordered regions | 1% |
What pLDDT means and how to read it
Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades, such as regulation of postsynaptic neurotransmitter release (PubMed:29925951, PubMed:33408414, PubMed:39103320). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:39103320). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:39103320). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (By similarity). Both GDP release and GTP hydrolysis are modulated by…
G proteins are composed of 3 units; alpha, beta and gamma (PubMed:29925951, PubMed:39103320, PubMed:34685729). The alpha chain contains the guanine nucleotide binding site (PubMed:29925951, PubMed:39103320). Forms a complex with GNB1 and GNG3 (PubMed:34685729). Forms a complex with GNB1 and GNG2; this complex associates with Mu-type opioid receptor OPRM1, D(2) dopamine receptor DRD2, neurotensin…
Cell membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 9BSB | EM | 2.32 Å | A=1-354 |
| 9VJE | EM | 2.47 Å | B=1-354 |
| 9K20 | EM | 2.65 Å | B=4-57, B=182-354 |
| 9VJ5 | EM | 2.69 Å | B=1-354 |
| 8E9X | EM | 2.7 Å | B=4-354 |
| 9PLO | EM | 2.74 Å | A=1-354 |
| 9VNF | EM | 2.74 Å | B=1-354 |
| 8YN7 | EM | 2.77 Å | A=4-354 |
| 8YN8 | EM | 2.77 Å | A=4-354 |
| 9KVP | EM | 2.79 Å | A=4-57, A=182-354 |
| 7W2Z | EM | 2.8 Å | A=182-354 |
| 8XXH | EM | 2.8 Å | A=4-57, A=182-354 |
| 8DZQ | EM | 2.82 Å | B=1-354 |
| 9VMY | EM | 2.86 Å | B=1-354 |
| 8FN1 | EM | 2.88 Å | B=1-57, B=182-354 |
| 8I95 | EM | 2.88 Å | A=4-57, A=182-354 |
| 9KV6 | EM | 2.88 Å | A=4-57, A=182-354 |
| 6K41 | EM | 2.9 Å | A=1-354 |
| 7D77 | EM | 2.9 Å | A=4-354 |
| 9DYE | EM | 2.9 Å | A=1-354 |
Showing 20 of 83 experimental structures (best resolution first).
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