P0ADY7: Large ribosomal subunit protein uL16 (rplP)

Large ribosomal subunit protein uL16 (rplP) is a 136-residue protein from Escherichia coli (strain K12). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P0ADY7.

Gene
rplP
Organism
Escherichia coli (strain K12)
Length
136 residues
Mean pLDDT
96.4
Model
AF-P0ADY7-F1 v6
Model created
1 Aug 2025
PDB structures
551

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Model confidence (pLDDT)

The mean pLDDT of this model is 96.4 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.

pLDDT bandMeaningShare of residues
Above 90Very high: backbone and side chains are usually accurate98%
70 to 90Confident: backbone generally right2%
50 to 70Low: treat with caution1%
Below 50Very low: often disordered regions0%

What pLDDT means and how to read it

Function

Binds directly to 23S ribosomal RNA and is located at the A site of the peptidyltransferase center. It contacts the A and P site tRNAs (PubMed:8524654). One of the last ribosomal proteins to be assembled in the 50S subunit (PubMed:33639093). The simultaneous presence of uL16 and bL36 probably triggers ObgE's GTPase activity and eventual dissociation from the mature 50S ribosomal subunit (PubMed:33639093). It has an essential role in subunit assembly (PubMed:3298242)

Subunit structure

Part of the 50S ribosomal subunit (PubMed:10094780, PubMed:12809609, PubMed:16272117, PubMed:24844575, PubMed:25310980, PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:786730, PubMed:33639093, PubMed:38842932). Cross-links to the A and P site tRNAs (PubMed:8524654). Interacts with assembly factor DarP(YjgA) (PubMed:38842932)

Experimental structures in the PDB

Compare the prediction with experimentally determined structures of the same protein:

PDB IDMethodResolutionChains and residues
8B0XEM1.55 Ål=1-136
9Q87EM1.55 Ål=1-136
8CGKEM1.64 Ål=1-136
8CGVEM1.66 Ål=1-136
8CEUEM1.83 Ål=1-136
8FTOEM1.85 Ål=1-136
8CAMEM1.86 Ål=1-136
9GHEEM1.87 Ål=1-136
9D89EM1.95 Ål=1-136
8AYEEM1.96 Ål=1-136
7K00EM1.98 Ål=1-136
8CGDEM1.98 Ål=1-136
8QOAEM2.0 Ål=1-136
8G6WEM2.02 Ål=1-136
8BILEM2.04 ÅJ=1-136
8BIMEM2.04 ÅJ=1-136
8G6YEM2.09 Ål=1-136
4YBBX-ray2.1 ÅCN/DN=1-136
6XZ7EM2.1 ÅM=1-136
7QQ3EM2.1 ÅU=1-136

Showing 20 of 551 experimental structures (best resolution first).

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