Ubiquitin-activating enzyme E1 1 (UBA1) is a 1024-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P22515.
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The mean pLDDT of this model is 93.6 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 86% |
| 70 to 90 | Confident: backbone generally right | 12% |
| 50 to 70 | Low: treat with caution | 1% |
| Below 50 | Very low: often disordered regions | 1% |
What pLDDT means and how to read it
E1 ubiquitin-activating enzyme that catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (By similarity). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:18662542, PubMed:1989885, PubMed:24816100, PubMed:35970836). The RSP5-UBA1-UBC5 ubiquitin ligase complex ubiquitinates RPO21 forming 'Lys-63'-linked polyubiquitin chains (PubMed:19920177)
Monomer (PubMed:24816100). Binds two ubiquitin chains simultaneously (PubMed:24816100). Component of the RSP5-UBA1-UBC5 ubiquitin ligase complex composed of E3 RSP5, E1 UBA1 and E2 UBC5 (Probable). Interacts with the E2 ubiquitin-conjugating enzyme UBC4 (By similarity)
Cytoplasm, Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 7ZH9 | X-ray | 1.72 Å | A=1-1024 |
| 6ZQH | X-ray | 2.03 Å | A/C=1-1024 |
| 6NYA | X-ray | 2.06 Å | A/D=11-1024 |
| 5TR4 | X-ray | 2.2 Å | A/C=9-1024 |
| 5L6H | X-ray | 2.3 Å | A/C=1-1024 |
| 6ZHT | X-ray | 2.3 Å | C=25-1024 |
| 6ZHS | X-ray | 2.35 Å | A=1-1024 |
| 4NNJ | X-ray | 2.4 Å | A/C=9-1024 |
| 5L6J | X-ray | 2.68 Å | A/C=1-1024 |
| 3CMM | X-ray | 2.7 Å | A/C=10-1024 |
| 5L6I | X-ray | 2.76 Å | A/C=1-1024 |
| 6ZHU | X-ray | 3.18 Å | A/C/E/G=1-1024 |
| 7K5J | X-ray | 3.42 Å | A/C/D/G/I/K/S/U=11-1024 |
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