Nucleosome assembly protein (NAP1) is a 417-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P25293.
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The mean pLDDT of this model is 77.9 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 51% |
| 70 to 90 | Confident: backbone generally right | 15% |
| 50 to 70 | Low: treat with caution | 15% |
| Below 50 | Very low: often disordered regions | 19% |
What pLDDT means and how to read it
Histone H2A-H2B chaperone that together with karyopherin KAP114 co-chaperones histone H2A-H2B dimers into the nucleus; subsequent binding of RAN(GTP) GSP1 to this complex promotes specific and accurate deposition of H2A-H2B into nucleosomes (PubMed:18086883, PubMed:2016313, PubMed:27225933). Also acts as an assembly chaperone for the small ribosomal subunit protein eS6 (RPS6A and RPS6B), thereby functioning in 40S ribosomal subunit biogenesis (PubMed:31062022). May also chaperone the large ribosomal subunit proteins eL39 and eL42 (PubMed:31062022). Plays a role in the regulation of the cell cycle; regulates the localization and function of septins during mitosis, and the function of B-type…
Homodimer (PubMed:16432217, PubMed:18068721, PubMed:27225933, PubMed:39601790). Component of the GIN4 complex composed of at least BNI5, CDC3, CDC10, CDC11, CDC12, GIN4, NAP1 and SHS1 which forms a ring at the bud neck (PubMed:12058072). Interacts with karyopherin KAP114 (via C-terminus); the interaction is direct and for the nuclear import of histone H2A-H2B dimers and their assembly into…
Cytoplasm, Nucleus, Bud neck
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 9B3I | EM | 2.88 Å | E/F=3-417 |
| 2AYU | X-ray | 3.0 Å | A=1-417 |
| 2Z2R | X-ray | 3.2 Å | A/B=74-365 |
| 9B23 | EM | 3.2 Å | D/E=74-365 |
| 9B31 | EM | 3.2 Å | D/E=74-365 |
| 9B3F | EM | 3.54 Å | D/E=74-365 |
| 5G2E | X-ray | 6.7 Å | A/B/E/F/I/J/M/N/Q/R/U/V=74-372 |
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