CD40 ligand (CD40LG) is a 261-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P29965.
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The mean pLDDT of this model is 82.6 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 57% |
| 70 to 90 | Confident: backbone generally right | 20% |
| 50 to 70 | Low: treat with caution | 14% |
| Below 50 | Very low: often disordered regions | 9% |
What pLDDT means and how to read it
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity).…
Homotrimer (PubMed:11676606, PubMed:8589998, PubMed:8626375). Interacts with isoform 3 of CD28 (PubMed:15067037). CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (PubMed:31331973, PubMed:8626375). Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (PubMed:31331973)
Cell membrane, Cell surface, Secreted
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 6W9G | X-ray | 1.82 Å | A/B/C=116-261 |
| 1ALY | X-ray | 2.0 Å | A=116-261 |
| 7SGM | X-ray | 2.0 Å | A/B/C=116-261 |
| 3LKJ | X-ray | 2.5 Å | A/B/C=121-261 |
| 6BRB | X-ray | 2.82 Å | A=120-261 |
| 1I9R | X-ray | 3.1 Å | A/B/C=116-261 |
| 9I5N | EM | 3.4 Å | C=119-261 |
| 3QD6 | X-ray | 3.5 Å | A/B/C/D/E/F=116-261 |
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