E3 ubiquitin-protein ligase RSP5 (RSP5) is a 809-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P39940.
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The mean pLDDT of this model is 74.6 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 36% |
| 70 to 90 | Confident: backbone generally right | 32% |
| 50 to 70 | Low: treat with caution | 11% |
| Below 50 | Very low: often disordered regions | 21% |
What pLDDT means and how to read it
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:15933713, PubMed:19920177, PubMed:30893611, PubMed:7708685). Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2 (PubMed:8596462). The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the…
Component of the RSP5-BUL1/2 ubiquitin ligase complex composed of at least RSP5 and BUL1 or BUL2 (PubMed:8668140, PubMed:9931424). Component of the RSP5-UBA1-UBC5 ubiquitin ligase complex composed of E3 RSP5, E1 UBA1 and E2 UBC5 (Probable). Also forms a ternary complex with RUP1 and UBP2 (PubMed:15933713). Interacts (via WW domains) with LSB1 (PubMed:22000681). Interacts (via WW domains) with…
Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, actin patch
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5HPL | X-ray | 2.31 Å | A/B=430-809 |
| 3OLM | X-ray | 2.5 Å | A=384-809 |
| 4LCD | X-ray | 3.1 Å | A/B=383-809 |
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