Vacuolar protein sorting-associated protein 27 (VPS27) is a 622-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P40343.
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The mean pLDDT of this model is 66.8 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 20% |
| 70 to 90 | Confident: backbone generally right | 31% |
| 50 to 70 | Low: treat with caution | 15% |
| Below 50 | Very low: often disordered regions | 34% |
What pLDDT means and how to read it
Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane (PubMed:11416128, PubMed:11872141, PubMed:12055639, PubMed:12900393, PubMed:14581452, PubMed:1493335, PubMed:15086794, PubMed:15107463, PubMed:15166140, PubMed:17135292, PubMed:18508771, PubMed:3062374, PubMed:9265642). Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi (PubMed:11208109, PubMed:8649377, PubMed:9015300). Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC (PubMed:8649377). Might also function as an alternate…
Component of the ESCRT-0 complex composed of HSE1 and VPS27 (PubMed:12055639, PubMed:14581452, PubMed:15086794). Interacts with ENT3 and ENT5, the ESCRT-I subunits VPS23 and VPS28 and with the COPIb subunits SEC27, SEC28 and SEC33 (PubMed:12900393, PubMed:15107463, PubMed:17101773). May form a complex composed of VPS27, HSE1 and DOA1 (PubMed:18508771). Interacts with DOA1 (PubMed:18508771).…
Endosome membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 1VFY | X-ray | 1.15 Å | A=163-231 |
| 1O06 | X-ray | 1.45 Å | A=301-320 |
| 3R42 | X-ray | 1.87 Å | B=445-453 |
| 6NJG | X-ray | 2.35 Å | B=256-278 |
| 2PJW | X-ray | 3.01 Å | V=348-438 |
| 1Q0V | NMR | A=250-329 | |
| 1Q0W | NMR | A=255-278 | |
| 2KDI | NMR | A=258-277 |
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