Mitogen-activated protein kinase 8 (MAPK8) is a 427-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P45983.
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The mean pLDDT of this model is 82.4 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 64% |
| 70 to 90 | Confident: backbone generally right | 15% |
| 50 to 70 | Low: treat with caution | 4% |
| Below 50 | Very low: often disordered regions | 17% |
What pLDDT means and how to read it
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed:28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed:18307971). Phosphorylates the replication…
Forms a complex with MAPK8IP1 and ARHGEF28 (By similarity). Found in a complex with SH3RF1, RAC1, MAP3K11/MLK3, MAP2K7/MKK7 and MAPK8IP1/JIP1. Found in a complex with SH3RF1, RAC2, MAP3K7/TAK1, MAP2K7/MKK7, MAPK8IP1/JIP1 and MAPK9/JNK2 (By similarity). Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4 (PubMed:15693750).…
Cytoplasm, Nucleus, Synapse
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 2XRW | X-ray | 1.33 Å | A=2-364 |
| 4QTD | X-ray | 1.5 Å | A=1-363 |
| 8R5E | X-ray | 1.7 Å | A=1-364 |
| 3ELJ | X-ray | 1.8 Å | A=1-364 |
| 4AWI | X-ray | 1.91 Å | A=1-364 |
| 4L7F | X-ray | 1.95 Å | A=7-362 |
| 3PZE | X-ray | 2.0 Å | A=7-364 |
| 8X5M | X-ray | 2.0 Å | A=2-364 |
| 4HYU | X-ray | 2.15 Å | A=1-363 |
| 4E73 | X-ray | 2.27 Å | A=1-363 |
| 4IZY | X-ray | 2.3 Å | A=1-363 |
| 4UX9 | X-ray | 2.34 Å | A/B/C/D=1-364 |
| 1UKH | X-ray | 2.35 Å | A=1-363 |
| 9FT9 | X-ray | 2.35 Å | A=5-364 |
| 4YR8 | X-ray | 2.4 Å | A/C/E/F=1-363 |
| 8PTA | X-ray | 2.41 Å | A/B/C=1-364 |
| 4HYS | X-ray | 2.42 Å | A=1-363 |
| 4G1W | X-ray | 2.45 Å | A=1-363 |
| 2XS0 | X-ray | 2.6 Å | A=1-379 |
| 3VUM | X-ray | 2.69 Å | A=1-364 |
Showing 20 of 39 experimental structures (best resolution first).
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