Spindle assembly checkpoint component MAD3 (MAD3) is a 515-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P47074.
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The mean pLDDT of this model is 65.9 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 11% |
| 70 to 90 | Confident: backbone generally right | 40% |
| 50 to 70 | Low: treat with caution | 18% |
| Below 50 | Very low: often disordered regions | 32% |
What pLDDT means and how to read it
Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20
Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20 (PubMed:10704439, PubMed:11726501, PubMed:15879521). Interacts with BUB3; the interaction is direct (PubMed:10704439, PubMed:11726501, PubMed:17227844)
Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 2I3T | X-ray | 2.8 Å | B/D/F/H=354-400 |
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