Outer kinetochore KNL1 complex subunit SPC105 (SPC105) is a 917-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P53148.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 53.7 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 4% |
| 70 to 90 | Confident: backbone generally right | 27% |
| 50 to 70 | Low: treat with caution | 18% |
| Below 50 | Very low: often disordered regions | 51% |
What pLDDT means and how to read it
Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:19893618, PubMed:22521787, PubMed:32997987, PubMed:35042402). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed:19893618). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect…
Component of the KNL1/SPC105 complex composed of SPC105 and KRE28 (PubMed:14565975, PubMed:16715078, PubMed:19893618, PubMed:32997987, PubMed:35042402, PubMed:9585415). Part of the outer kinetochore KMN network that includes the KNL1, MIS12 and NDC80 complexes (PubMed:14565975, PubMed:16715078, PubMed:19893618, PubMed:22521787, PubMed:32997987, PubMed:9585415). Interacts (via phosphorylated MELT…
Nucleus, Chromosome, centromere, kinetochore
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 4BL0 | X-ray | 1.95 Å | C/F=165-183 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.