DASH complex subunit DAM1 (DAM1) is a 343-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P53267.
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The mean pLDDT of this model is 58.8 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 15% |
| 70 to 90 | Confident: backbone generally right | 10% |
| 50 to 70 | Low: treat with caution | 29% |
| Below 50 | Very low: often disordered regions | 46% |
What pLDDT means and how to read it
Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerization to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase (PubMed:11698664, PubMed:11724818, PubMed:15664196, PubMed:16415853, PubMed:16777964, PubMed:17460120, PubMed:17643123, PubMed:9817759). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection…
Component of the DASH complex consisting of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with a stoichiometry of one copy of each subunit per complex (PubMed:11782438, PubMed:12925767, PubMed:15640796, PubMed:16715078, PubMed:17460120, PubMed:17643123, PubMed:24930965, PubMed:25236177). Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and…
Nucleus, Cytoplasm, cytoskeleton, spindle, Chromosome, centromere, kinetochore
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 8Q84 | EM | 3.15 Å | I/U/e=1-343 |
| 8G0Q | X-ray | 3.22 Å | B/D=252-305 |
| 8QAU | EM | 3.54 Å | E=1-343 |
| 8Q85 | EM | 3.97 Å | U=1-343 |
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