NEDD8-conjugating enzyme Ubc12 (UBE2M) is a 183-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P61081.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 91.4 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 85% |
| 70 to 90 | Confident: backbone generally right | 1% |
| 50 to 70 | Low: treat with caution | 10% |
| Below 50 | Very low: often disordered regions | 3% |
What pLDDT means and how to read it
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation
Interacts with UBA3 and RBX1. Interacts (N-terminally acetylated form) with (via DCUN1 domain) DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5 (PubMed:23201271, PubMed:28581483)
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3TDU | X-ray | 1.5 Å | E/F=1-15 |
| 3TDZ | X-ray | 2.0 Å | E/F=1-12 |
| 1Y8X | X-ray | 2.4 Å | A=27-183 |
| 1TT5 | X-ray | 2.6 Å | E/F=1-26 |
| 2NVU | X-ray | 2.8 Å | C=1-183 |
| 4P5O | X-ray | 3.11 Å | G/I=2-183 |
| 4GAO | X-ray | 3.28 Å | C/E/F/H=1-12 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.