Large ribosomal subunit protein uL5 (rplE) is a 182-residue protein from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q5SHQ0.
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The mean pLDDT of this model is 91.8 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 81% |
| 70 to 90 | Confident: backbone generally right | 18% |
| 50 to 70 | Low: treat with caution | 1% |
| Below 50 | Very low: often disordered regions | 1% |
What pLDDT means and how to read it
This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (forming bridge B1b) connecting the head of the 30S subunit to the top of the 50S subunit. The bridge itself contacts the P site tRNA and is implicated in movement during ribosome translocation. Also contacts the P site tRNA independently of the intersubunit bridge; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs
Part of the 50S ribosomal subunit; part of the 5S rRNA/uL5/uL18/bL25 (TL7) subcomplex; has also been isolated as a complex with 5S rRNA, bL25 (TL7) and DNA binding protein II. Forms a bridge to the 30S subunit in the 70S ribosome, contacting protein uS13; this bridge is straddled by the 5S rRNA. Contacts the P site tRNA
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 9NO7 | EM | 2.13 Å | G=1-182 |
| 4Y4O | X-ray | 2.3 Å | 1G/2G=1-182 |
| 8CVL | X-ray | 2.3 Å | 1G/2G=1-182 |
| 8FC6 | X-ray | 2.35 Å | 1G/2G=1-182 |
| 8VTW | X-ray | 2.35 Å | 1G/2G=1-182 |
| 4W2F | X-ray | 2.4 Å | BG/DG=1-182 |
| 6XHV | X-ray | 2.4 Å | 1G/2G=1-182 |
| 7RQA | X-ray | 2.4 Å | 1G/2G=1-182 |
| 7RQE | X-ray | 2.4 Å | 1G/2G=1-182 |
| 8CVJ | X-ray | 2.4 Å | 1G/2G=1-182 |
| 8VTU | X-ray | 2.4 Å | 1G/2G=1-182 |
| 8VTX | X-ray | 2.4 Å | 1G/2G=1-182 |
| 9MTP | X-ray | 2.4 Å | 1G/2G=1-182 |
| 10PX | X-ray | 2.45 Å | 1G/2G=1-182 |
| 7RQB | X-ray | 2.45 Å | 1G/2G=1-182 |
| 8FC4 | X-ray | 2.45 Å | 1G/2G=1-182 |
| 8G2A | X-ray | 2.45 Å | 1G/2G=1-182 |
| 9D0I | X-ray | 2.45 Å | 1G/2G=1-182 |
| 4Y4P | X-ray | 2.5 Å | 1G/2G=1-182 |
| 6XHW | X-ray | 2.5 Å | 1G/2G=1-182 |
Showing 20 of 269 experimental structures (best resolution first).
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