Calmodulin-dependent glutamylase SidJ (sidJ) is a 873-residue protein from Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q5ZTK6.
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The mean pLDDT of this model is 88.0 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 83% |
| 70 to 90 | Confident: backbone generally right | 3% |
| 50 to 70 | Low: treat with caution | 2% |
| Below 50 | Very low: often disordered regions | 12% |
What pLDDT means and how to read it
Glutamylase that mediates the covalent attachment of glutamate moieties to SdeA on one of the catalytic residues that is required for its mono-ADP-ribosyltransferase activity (PubMed:31330531, PubMed:31330532). In turn, inhibits SdeA ubiquitinating activity. Also glutamylates related SdeB, SdeC and SidE (PubMed:31123136, PubMed:31330531). Glutamylase activity only occurs in the host since it requires host calmodulin (PubMed:28497808, PubMed:31123136, PubMed:31330531, PubMed:31330532). May also reverse the SdeA-mediated substrate ubiquitination by cleaving the phosphodiester bond that links phosphoribosylated ubiquitin to protein substrates via its deubiquitinase activity (PubMed:28497808)
Interacts with host calmodulin/CALM1; this interaction is required for glutamylase activity
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 6OQQ | X-ray | 2.1 Å | A/C=1-873 |
| 7MIR | EM | 2.5 Å | A=97-851 |
| 6PLM | X-ray | 2.59 Å | A/B=97-853 |
| 6K4K | X-ray | 2.71 Å | A/B=1-873 |
| 7MIS | EM | 2.8 Å | A=97-851 |
| 7PPO | EM | 2.91 Å | C=99-873 |
| 6K4L | X-ray | 2.95 Å | A/B=1-873 |
| 6K4R | X-ray | 3.11 Å | A/B=1-873 |
| 7PQE | EM | 3.7 Å | C=99-873 |
| 6S5T | EM | 4.15 Å | A=1-873 |
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