5-cytosine rRNA methyltransferase NSUN4 (NSUN4) is a 384-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q96CB9.
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The mean pLDDT of this model is 91.3 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 87% |
| 70 to 90 | Confident: backbone generally right | 2% |
| 50 to 70 | Low: treat with caution | 3% |
| Below 50 | Very low: often disordered regions | 9% |
What pLDDT means and how to read it
Mitochondrial RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as rRNAs, mRNAs and some long non-coding RNAs (lncRNAs) (PubMed:21531335, PubMed:23022348, PubMed:39019044). Involved in mitochondrial ribosome small subunit (SSU) maturation by catalyzing methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU) (PubMed:21531335, PubMed:23022348). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled (PubMed:21531335, PubMed:23022348). In vitro can methylate 16S rRNA…
Heterodimer with MTERFD2/MTERF4; this interaction seems to be required for NSUN4 recruitment to the mitochondrial large ribosomal subunit
Mitochondrion
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 4FZV | X-ray | 2.0 Å | A=26-384 |
| 7OF0 | EM | 2.2 Å | C=1-384 |
| 7O9M | EM | 2.5 Å | A1=1-384 |
| 7OF7 | EM | 2.5 Å | C=1-384 |
| 7OF3 | EM | 2.7 Å | C=1-384 |
| 4FP9 | X-ray | 2.9 Å | A/C/D/F=26-384 |
| 7ODR | EM | 2.9 Å | x=1-384 |
| 7OF5 | EM | 2.9 Å | C=1-384 |
| 8QSJ | EM | 3.0 Å | x=1-384 |
| 8PK0 | EM | 3.03 Å | x=1-384 |
| 7O9K | EM | 3.1 Å | A1=1-384 |
| 7ODS | EM | 3.1 Å | x=1-384 |
| 7ODT | EM | 3.1 Å | x=1-384 |
| 7OIC | EM | 3.1 Å | x=1-384 |
| 7PD3 | EM | 3.4 Å | x=1-384 |
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