(E3-independent) E2 ubiquitin-conjugating enzyme (UBE2O) is a 1292-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9C0C9.
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The mean pLDDT of this model is 65.6 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 32% |
| 70 to 90 | Confident: backbone generally right | 19% |
| 50 to 70 | Low: treat with caution | 8% |
| Below 50 | Very low: often disordered regions | 40% |
What pLDDT means and how to read it
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular…
Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain). Interacts with UBR2
Cytoplasm, Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 9QUG | X-ray | 1.8 Å | A=920-1292 |
| 9MC5 | EM | 3.29 Å | B=1-1292 |
| 7UN6 | EM | 3.3 Å | A=1-1292 |
| 9MC4 | EM | 3.32 Å | B=1-1292 |
| 9MC6 | EM | 3.32 Å | B=1-1292 |
| 9MC7 | EM | 3.34 Å | B=928-1292 |
| 9MC9 | EM | 3.37 Å | B=928-1292 |
| 9MCB | EM | 3.42 Å | B=928-1292 |
| 7UN3 | EM | 3.5 Å | A/D=1-1292 |
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