Ceramide synthase 5 (Cers5) is a 414-residue protein from Mus musculus. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9D6K9.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 82.1 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 64% |
| 70 to 90 | Confident: backbone generally right | 16% |
| 50 to 70 | Low: treat with caution | 3% |
| Below 50 | Very low: often disordered regions | 17% |
What pLDDT means and how to read it
Ceramide synthase that catalyzes the transfer of the fatty acyl chain from fatty acyl-CoA to a sphingoid base, with high selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16:0-CoA) (PubMed:12912983, PubMed:15823095, PubMed:16100120, PubMed:17609214, PubMed:17977534, PubMed:26853464, PubMed:38961186). Can use different sphingoid bases as substrates for ceramide synthesis, such as sphinganine in de novo synthesis, and sphing-4E-enine (sphingosine), (4R)-hydroxysphinganine (phytosphingosine) or sphinga-4E,14Z-dienine in salvage pathways (By similarity) (PubMed:12912983, PubMed:15772421, PubMed:15823095, PubMed:17977534). Other fatty acyl-CoAs such as long-chain saturated stearoyl-CoA…
Interacts with PAQR4; the interaction regulates the stability and activity of CERS5 and is inhibited in presence of ceramides
Endoplasmic reticulum membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5E8O | X-ray | 1.98 Å | C/F=379-387 |
| 5E8P | X-ray | 2.0 Å | C/F=379-387 |
| 5E8N | X-ray | 2.25 Å | C/F/I/L=379-387 |
| 2CQX | NMR | A=77-135 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.