Iron-sulfur cluster assembly enzyme ISCU (ISCU) is a 167-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9H1K1.
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The mean pLDDT of this model is 85.2 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 71% |
| 70 to 90 | Confident: backbone generally right | 5% |
| 50 to 70 | Low: treat with caution | 20% |
| Below 50 | Very low: often disordered regions | 4% |
What pLDDT means and how to read it
Mitochondrial scaffold protein, of the core iron-sulfur cluster (ISC) assembly complex, that provides the structural architecture on which the [2Fe-2S] clusters are assembled (PubMed:34824239). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the…
Homodimer; Tyr-35-mediated dimerization of two iron- and sulfide-containing ISCU subunit bind to the cysteine desulfurase complex (PubMed:31101807, PubMed:34824239). Component of the mitochondrial core iron-sulfur cluster (ISC) complex composed of NFS1, LYRM4, NDUFAB1, ISCU, FXN, and FDX2; this complex is an heterohexamer containing two copies of each monomer (Probable) (PubMed:21298097,…
Mitochondrion, Cytoplasm, Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 6UXE | X-ray | 1.57 Å | D=35-167 |
| 6W1D | X-ray | 1.79 Å | D=35-167 |
| 6WIH | X-ray | 1.9 Å | D=35-167 |
| 6WI2 | X-ray | 1.95 Å | D=35-167 |
| 8TVT | X-ray | 2.0 Å | D=35-167 |
| 8RMC | EM | 2.26 Å | D/H=35-167 |
| 8RMF | EM | 2.33 Å | D/H=35-167 |
| 8RMG | EM | 2.46 Å | D/H=35-167 |
| 8PK8 | EM | 2.49 Å | D=35-167 |
| 8RME | EM | 2.49 Å | D/H=35-167 |
| 7RTK | X-ray | 2.5 Å | D=35-167 |
| 8RMD | EM | 2.52 Å | D/H=35-167 |
| 8PK9 | EM | 2.58 Å | D=35-167 |
| 8PKA | EM | 2.75 Å | D=35-167 |
| 5WKP | X-ray | 3.15 Å | D/H=38-167 |
| 6NZU | EM | 3.2 Å | D/H=34-157 |
| 5WLW | X-ray | 3.32 Å | D/H=38-167 |
| 5KZ5 | EM | 14.3 Å | a/b/c/d/e/f/g/h/i/j/k/l=50-167 |
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