Cysteine desulfurase (NFS1) is a 457-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9Y697.
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The mean pLDDT of this model is 88.8 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 81% |
| 70 to 90 | Confident: backbone generally right | 5% |
| 50 to 70 | Low: treat with caution | 2% |
| Below 50 | Very low: often disordered regions | 13% |
What pLDDT means and how to read it
Cysteine desulfurase, of the core iron-sulfur cluster (ISC) assembly complex, that catalyzes the desulfuration of L-cysteine to L-alanine, as component of the cysteine desulfurase complex, leading to the formation of a cysteine persulfide intermediate at the active site cysteine residue and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (PubMed:18650437, PubMed:29097656, PubMed:31101807). The persulfide is then transferred on the flexible Cys loop from the catalytic site of NFS1 to the surface of NFS1 (PubMed:29097656). After the NFS1-linked persulfide sulfur is transferred to one of the conserved Cys residues of the scaffold, a reaction assisted by FXN (By…
Homodimer (PubMed:29097656, PubMed:31101807). Component of the mitochondrial core iron-sulfur cluster (ISC) complex composed of NFS1, LYRM4, NDUFAB1, ISCU, FXN, and FDX2; this complex is a heterohexamer containing two copies of each monomer (Probable). Component of cyteine desulfurase complex composed of NFS1, LYRM4 and NDUFAB1; this complex contributes to the activation of cysteine desulfurase…
Mitochondrion, Cytoplasm, cytosol, Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 6UXE | X-ray | 1.57 Å | A=56-457 |
| 6W1D | X-ray | 1.79 Å | A=56-457 |
| 6WIH | X-ray | 1.9 Å | A=56-457 |
| 6WI2 | X-ray | 1.95 Å | A=56-457 |
| 8TVT | X-ray | 2.0 Å | A=56-454 |
| 8RMC | EM | 2.26 Å | A/E=56-457 |
| 8RMF | EM | 2.33 Å | A/E=56-457 |
| 8RMG | EM | 2.46 Å | A/E=56-457 |
| 8PK8 | EM | 2.49 Å | A=56-457 |
| 8RME | EM | 2.49 Å | A/E=56-457 |
| 7RTK | X-ray | 2.5 Å | A=56-457 |
| 8RMD | EM | 2.52 Å | A/E=56-457 |
| 8PK9 | EM | 2.58 Å | A=56-457 |
| 5WGB | X-ray | 2.75 Å | A=56-457 |
| 8PKA | EM | 2.75 Å | A=56-457 |
| 5USR | X-ray | 3.09 Å | A/C/E/G=56-457 |
| 5WKP | X-ray | 3.15 Å | A/E=56-457 |
| 6NZU | EM | 3.2 Å | A/E=55-457 |
| 5WLW | X-ray | 3.32 Å | A/E=56-457 |
| 5KZ5 | EM | 14.3 Å | 1/2/3/4/M/N/O/P/Q/R/S/T=67-457 |
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