10JI: Human Argonaute2-miR200b RISC

Structure of Human Argonaute2-miR200b RISC bound to 14nt long target in complex with kinase CK1alpha. Determined by electron microscopy at 3.7 Å resolution. Released 23 Sept 2026.

Method
Electron microscopy
Resolution
3.7 Å
Organism
Homo sapiens
Chains
4
Atoms
9,750
Mol. weight
147.7 kDa
Released
23 Sept 2026

Explore 10JI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

10JI contains 52 α-helices and 70 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 37 helices, 51 β-strands

ElementResiduesLengthSheet
α-helix25-284
β-strand36-4271
β-strand44-4632
β-strand47-4823
β-strand53-6194
α-helix68-8114
α-helix921
β-strand93-9424
β-strand99-10244
β-strand113-11974
β-strand126-139144
α-helix140-1489
α-helix156-17318
β-strand175-17732
β-strand180-18232
β-strand19112
β-strand196-209142
β-strand212-225142
β-strand22915
α-helix230-2389
α-helix243-2453
α-helix249-2513
α-helix252-26211
β-strand266-26945
β-strand278-28035
β-strand281-28446
β-strand293-29537
β-strand305-30737
α-helix308-3147
β-strand327-33046
β-strand339-34026
α-helix341-3433
β-strand344-34635
α-helix3471
α-helix350-3523
α-helix355-3573
α-helix358-36811
α-helix372-38514
α-helix392-3976
β-strand400-40123
β-strand406-41271
α-helix413-4153
β-strand418-41928
β-strand42119
β-strand427-42828
β-strand431110
β-strand434110
β-strand44119
β-strand444111
β-strand448112
β-strand451-455513
α-helix464-48118
β-strand485112
β-strand491-494413
α-helix498-5003
α-helix501-51111
β-strand517-521513
α-helix527-5348
α-helix535-5395
β-strand544-547413
α-helix549-5535
α-helix557-57014
β-strand574111
β-strand577-57828
α-helix585-5884
β-strand591-59991
α-helix6091
β-strand610-61781
β-strand625-63281
α-helix642-65716
β-strand663-66971
α-helix673-6753
α-helix676-69419
β-strand701-70881
β-strand715-71731
α-helix720-7223
β-strand724114
β-strand729114
α-helix730-7312
β-strand734-73631
β-strand747-75151
β-strand763-77081
α-helix776-78611
β-strand795110
α-helix801-81717
α-helix839-8457
α-helix847-8493
α-helix852-8543
Chain B: 15 helices, 19 β-strands
ElementResiduesLengthSheet
β-strand17-19315
β-strand24-25216
β-strand30-32316
β-strand34-36315
β-strand42115
β-strand44-48516
α-helix56-6712
α-helix74-752
β-strand76-79416
β-strand82117
β-strand85117
β-strand86-90516
β-strand96118
α-helix97-1048
α-helix110-12920
β-strand132-133219
α-helix139-1413
β-strand142-144318
α-helix147-1493
β-strand153-155318
β-strand162-163219
β-strand165120
β-strand172120
α-helix173-1753
β-strand177121
α-helix190-1945
β-strand196121
α-helix197-1982
α-helix201-21515
α-helix229-24214
α-helix245-2495
α-helix255-26511
α-helix274-28815
α-helix297-3048

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Protein argonaute-2Aprotein860Homo sapiensQ9UKV8 (AlphaFold model)
Casein kinase I isoform alphaBprotein336Homo sapiensP48729 (AlphaFold model)
RNA (5'-r(p*up*ap*ap*up*ap*cp*up*gp*cp*cp*up*gp*gp*up*ap*ap*up*gp*ap*up*gp*a)-3')CRNA22Homo sapiens
RNA (5'-r(p*ap*cp*cp*up*ap*up*cp*ap*gp*up*ap*up*up*a)-3')DRNA14Homo sapiens
Sequence of entity 1 (A), FASTA
>10JI_1 Protein argonaute-2 (chains A)
AMYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYEL
DIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTL
PGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGR
SFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLD
FKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQE
SGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDN
QTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSIL
YGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISR
DAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTV
LGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVT
HPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRF
KPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTD
KNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDEL
QILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDH
QALAKAVQVHQDTLRTMYFA
Sequence of entity 2 (B), FASTA
>10JI_2 Casein kinase I isoform alpha (chains B)
ASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYES
KLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMI
SRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNL
TGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKM
STPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTML
KQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF
Sequence of entity 3 (C), FASTA
>10JI_3 RNA (5'-R(P*UP*AP*AP*UP*AP*CP*UP*GP*CP*CP*UP*GP*GP*UP*AP*AP*UP*GP*AP*UP*GP*A)-3') (chains C)
UAAUACUGCCUGGUAAUGAUGA
Sequence of entity 4 (D), FASTA
>10JI_4 RNA (5'-R(P*AP*CP*CP*UP*AP*UP*CP*AP*GP*UP*AP*UP*UP*A)-3') (chains D)
ACCUAUCAGUAUUA

Primary citation

Structure of Human Argonaute2-miR200b RISC bound to 14nt long target in complex with kinase CK1alpha. Garg, A., Joshua-Tor, L. To be published.

Other PDB entries of the same protein (UniProt Q9UKV8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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