10OZ: PDE5 PDE6 chimera

Structure of PDE5 PDE6 chimera. Determined by electron microscopy at 3.06 Å resolution. Released 6 May 2026.

Method
Electron microscopy
Resolution
3.06 Å
Organism
Bos taurus
Chains
4
Atoms
12,147
Mol. weight
208.96 kDa
Ligands
ZN, MG, PCG, VIA
Released
6 May 2026

Explore 10OZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

10OZ contains 72 α-helices and 34 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 33 helices, 16 β-strands

ElementResiduesLengthSheet
α-helix50-6516
α-helix71-8616
β-strand88-99121
β-strand102-10981
α-helix118-1214
β-strand12211
α-helix125-1273
β-strand130-13231
α-helix136-1449
β-strand148-15031
α-helix162-1676
β-strand174-18181
β-strand184-194111
α-helix202-24544
α-helix252-26211
β-strand269-27792
β-strand30313
β-strand30913
β-strand312-31982
β-strand325-33062
α-helix343-3508
β-strand35314
β-strand354-35742
β-strand381-38772
β-strand396-40382
α-helix408-4092
α-helix411-42616
α-helix428-45427
α-helix459-46911
α-helix493-50614
α-helix509-5124
α-helix517-53014
α-helix540-55516
α-helix559-5624
α-helix565-57612
α-helix587-5926
α-helix596-5994
α-helix605-61915
α-helix631-64616
α-helix650-66516
α-helix674-68916
α-helix691-6933
α-helix697-72226
α-helix732-7376
α-helix738-7458
α-helix746-7505
α-helix751-76010
α-helix762-7643
α-helix765-78218
Chain B: 36 helices, 16 β-strands
ElementResiduesLengthSheet
α-helix51-6515
α-helix71-8616
β-strand88-99125
β-strand102-10985
α-helix118-1214
β-strand12215
α-helix125-1273
β-strand130-13235
α-helix136-1449
β-strand148-15035
α-helix162-1676
β-strand174-18185
β-strand184-194115
α-helix202-24544
α-helix252-26110
α-helix264-2674
β-strand269-27796
α-helix2781
β-strand30317
β-strand30917
β-strand312-31986
β-strand325-33066
α-helix343-3508
β-strand35318
β-strand354-35746
α-helix359-3613
β-strand381-38886
β-strand394-403106
α-helix408-4092
α-helix411-45444
α-helix459-46911
α-helix493-50614
α-helix509-5124
α-helix517-53014
α-helix540-55516
α-helix560-5623
α-helix565-57612
α-helix587-5926
α-helix596-6005
α-helix605-61814
α-helix631-64616
α-helix650-66516
α-helix674-68916
α-helix691-6933
α-helix697-71721
α-helix718-7225
α-helix735-7373
α-helix738-7458
α-helix746-7516
α-helix752-7609
α-helix762-7643
α-helix765-78218
Chain C: 2 helices, 1 β-strand
ElementResiduesLengthSheet
α-helix27-293
β-strand3118
α-helix32-332
Chain D: 1 helix, 1 β-strand
ElementResiduesLengthSheet
β-strand3114
α-helix34-374

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
cGMP-specific 3',5'-cyclic phosphodiesterase PDE5-PDE6 chimeraA, Bprotein800Bos taurusP16586 (AlphaFold model), Q28156 (AlphaFold model)
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gammaC, Dprotein107Bos taurusP04972 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>10OZ_1 cGMP-specific 3',5'-cyclic phosphodiesterase PDE5-PDE6 chimera (chains A, B)
MGEISQETVEKYLEANPQFAKEYFNRKLQVEVPSGGAQAPASASFPGRTLAEEAALYLEL
LEVLLEEAGSVELAAHRALQRLAQLLQADRCSMFLCRARNGTPEVASKLLDVTPTSKFED
NLVVPDREAVFPLDVGIVGWVAHTKKTFNVPDVKKNSHFSDFMDKQTGYVTRNLLATPIV
MGKEVLAVFMAVNKVDASEFSKQDEEVFSKYLSFVSIILKLHHTNYLYNIESRRSQILMW
SANKVFEELTDVERQFHKALYTVRTYLNCERYSIGLLDMTKEKEFYDEWPVKLGEVEPYK
GPKTPDGREVIFYKIIDYILHGKEEIKVIPTPPMDHWTLISGLPTYVAENGFICNMLNAP
ADEYFTFQKGPVDETGWVIKNVLSLPIVNKKEDIVGVATFYNRKDGKPFDEYDEHIAETL
TQFLGWSLLNTDTYEKMNKLMAKQMVTLEVLSYHASAAEEETRELQSLAAAVVPSAQTLK
ITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCKWILSVKKNYRKNVAYHNW
RHAFNTAQCMFAALKAGKIQKRLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQL
YCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEF
FELIMKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERK
ELNIEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQA
LAEQQEKTLINGESSQTKRN
Sequence of entity 2 (C, D), FASTA
>10OZ_2 Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma (chains C, D)
MGSSHHHHHHSSGLVPRGSHMNLEPPKAEIRSATRVMGGPVTPRKGPPKFKQRQTRQFKS
KPPKKGVQGFGDDIPGMEGLGTDITVICPWEAFNHLELHELAQYGII

Ligands and cofactors

IDNameFormulaCopies
ZNZinc ionZn2
MGMagnesium ionMg2
PCGCyclic guanosine monophosphateC10 H12 N5 O7 P2
VIA5-{2-ethoxy-5-[(4-methylpiperazin-1-yl)sulfonyl]phenyl}-1-methyl-3-propyl-1H,6H…C22 H30 N6 O4 S2

Primary citation

Structural basis of phosphodiesterase-5 conformational organization revealed by a PDE6/PDE5 chimera. Srivastava, D., Singh, S., Yu, C. et al. J Biol Chem (2026) 302:111467-111467. DOI 10.1016/j.jbc.2026.111467 · PubMed

Other PDB entries of the same protein (UniProt P16586 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 10OZ directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.