1BXI: Protein

Crystal structure of the escherichia coli colicin E9 DNase domain with its cognate immunity protein IM9. Determined by X-ray diffraction at 2.05 Å resolution. Released 4 Oct 1999.

Method: X-ray diffraction
Resolution: 2.05 Å
Organism: Escherichia coli
Chains: 2
Atoms: 1,794
Mol. weight: 24.89 kDa
Ligands: PO4, NI
Released: 4 Oct 1999

Explore 1BXI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1BXI contains 17 α-helices and 13 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	7-9	3
α-helix	12-23	12
α-helix	30-44	15
α-helix	51-54	4
α-helix	64-77	14

Chain B: 12 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	4-6	3
β-strand	9-10	2	1
β-strand	12	1	2
β-strand	16	1	3
α-helix	22-25	4
β-strand	32-33	2	4
α-helix	34	1
β-strand	35	1	3
α-helix	36-42	7
β-strand	46-47	2	1
α-helix	50-62	13
α-helix	65-68	4
α-helix	73-80	8
α-helix	83-85	3
β-strand	86	1	5
α-helix	87-88	2
α-helix	89-91	3
β-strand	93	1	6
β-strand	96	1	6
β-strand	98	1	5
β-strand	100-103	4	4
β-strand	115	1	2
α-helix	116-118	3
β-strand	119-122	4	4
α-helix	124-129	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein (colicin E9 immunity protein)	A	protein	86	Escherichia coli	P13479 (AlphaFold model)
Protein (colicin E9)	B	protein	134	Escherichia coli	P09883 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1BXI_1 PROTEIN (COLICIN E9 IMMUNITY PROTEIN) (chains A)
MELKASISDYTEAEFLQLVTTICNADTSSEEELVKLVTHFEEMTEHPSGSDLIYYPKEGD
DDSPSGIVNTVQQWRAANGKSGFKQG

Sequence of entity 2 (B), FASTA

>1BXI_2 PROTEIN (COLICIN E9) (chains B)
MESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKAVWEE
VSKDPELSKNLNPSNKSSVSKGYSPFTPKNQQVGGRKVYELHHDKPISQGGEVYDMDNIR
VTTPKRHIDIHRGK

Ligands and cofactors

ID	Name	Formula	Copies
PO4	Phosphate ion	O4 P	1
NI	Nickel (II) ion	Ni	1

Primary citation

Crystal Structure of the E.Coli Colicin E9 DNase Domain With its Cognate Immunity Protein Im9. Kuhlmann, U.C. Thesis (1998). PubMed

Other PDB entries of the same protein (UniProt P13479 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1FR2 1.6 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9(E41A)
2GYK 1.6 Å, Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity…
2GZJ 1.6 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (D51A)
2VLN 1.6 Å, N75A mutant of E9 DNase domain in complex with Im9
2VLQ 1.6 Å, F86A mutant of E9 DNase domain in complex with Im9
1EMV 1.7 Å, Crystal structure of colicin E9 DNase domain with its cognate immunity protein IM9 (1.7 Å)
2GZG 1.7 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (Y55F)
2GZI 1.7 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (V34A)
2GZF 1.75 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y54F)
2GZE 1.8 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y55A)
2VLO 1.8 Å, K97A mutant of E9 DNase domain in complex with Im9
2VLP 2.0 Å, R54A mutant of E9 DNase domain in complex with Im9

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