1E0H: Inhibitor Protein Im9

Inhibitor Protein Im9 bound to its partner E9 DNase. Determined by solution NMR. Released 30 Oct 2000.

Method: Solution NMR
Organism: ESCHERICHIA COLI
Chains: 1
Atoms: 673
Mol. weight: 9.59 kDa
Released: 30 Oct 2000

Explore 1E0H in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1E0H contains 5 α-helices and 2 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	7-9	3
β-strand	11	1	1
α-helix	12-22	11
α-helix	31-43	13
α-helix	49-51	3
α-helix	66-77	12
β-strand	84	1	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Immunity protein for colicin E9	A	protein	86	ESCHERICHIA COLI	P13479 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1E0H_1 IMMUNITY PROTEIN FOR COLICIN E9 (chains A)
MELKHSISDYTEAEFLQLVTTICNADTSSEEELVKLVTHFEEMTEHPSGSDLIYYPKEGD
DDSPSGIVNTVKQWRAANGKSGFKQG

Primary citation

NMR investigation of the interaction of the inhibitor protein Im9 with its partner DNase. Boetzel, R., Czisch, M., Kaptein, R. et al. Protein Sci (2000) 9:1709-1718. DOI 10.1110/ps.9.9.1709 · PubMed

Other PDB entries of the same protein (UniProt P13479 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1FR2 1.6 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9(E41A)
2GYK 1.6 Å, Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity…
2GZJ 1.6 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (D51A)
2VLN 1.6 Å, N75A mutant of E9 DNase domain in complex with Im9
2VLQ 1.6 Å, F86A mutant of E9 DNase domain in complex with Im9
1EMV 1.7 Å, Crystal structure of colicin E9 DNase domain with its cognate immunity protein IM9 (1.7 Å)
2GZG 1.7 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (Y55F)
2GZI 1.7 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (V34A)
2GZF 1.75 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y54F)
2GZE 1.8 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y55A)
2VLO 1.8 Å, K97A mutant of E9 DNase domain in complex with Im9
2VLP 2.0 Å, R54A mutant of E9 DNase domain in complex with Im9

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