1EJ3: Aequorin

Crystal structure of aequorin. Determined by X-ray diffraction at 2.3 Å resolution. Released 31 May 2000.

Method: X-ray diffraction
Resolution: 2.3 Å
Organism: Aequorea aequorea
Chains: 2
Atoms: 3,233
Mol. weight: 44.23 kDa
Ligands: CZH
Released: 31 May 2000

Explore 1EJ3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1EJ3 contains 23 α-helices and 8 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	10-23	14
β-strand	30-31	2	1
α-helix	33-46	14
α-helix	52-68	17
β-strand	77-78	2	1
α-helix	79-98	20
α-helix	101-103	3
α-helix	104-116	13
β-strand	123-124	2	2
α-helix	126-136	11
α-helix	142-152	11
β-strand	160-161	2	2
α-helix	162-169	8
α-helix	170-175	6
α-helix	178-180	3

Chain B: 12 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	10-23	14
β-strand	30-32	3	3
α-helix	33-42	10
α-helix	43-47	5
α-helix	52-68	17
β-strand	76-78	3	3
α-helix	79-98	20
α-helix	101-103	3
α-helix	104-116	13
β-strand	123-124	2	4
α-helix	126-136	11
α-helix	142-151	10
β-strand	160-161	2	4
α-helix	162-169	8
α-helix	170-175	6
α-helix	178-180	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Aequorin	A, B	protein	191	Aequorea aequorea	P02592 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>1EJ3_1 AEQUORIN (chains A, B)
ANSKLTSDFDNPRWIGRHKHMFNFLDVNHNGKISLDEMVYKASDIVINNLGATPEQAKRH
KDAVEAFFGGAGMKYGVETDWPAYIEGWKKLATDELEKYAKNEPTLIRIWGDALFDIVDK
DQNGAITLDEWKAYTKAAGIIQSSEDCEETFRVCDIDESGQLDVDEMTRQHLGFWYTMDP
ACEKLYGGAVP

Ligands and cofactors

ID	Name	Formula	Copies
CZH	C2-hydroperoxy-coelenterazine	C26 H21 N3 O5	2

Primary citation

The crystal structure of the photoprotein aequorin at 2.3 A resolution. Head, J.F., Inouye, S., Teranishi, K. et al. Nature (2000) 405:372-376. DOI 10.1038/35012659 · PubMed

Other PDB entries of the same protein (UniProt P02592 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1UHK 1.6 Å, Crystal structure of n-aequorin
1UHH 1.8 Å, Crystal structure of cp-aequorin
1UHI 1.8 Å, Crystal structure of i-aequorin
1UHJ 1.8 Å, Crystal structure of br-aequorin
7EG3 2.09 Å, Crystal structure of the apoAequorin complex with (S)-HM-daCTZ
5ZAB 2.15 Å, Crystal structure of cf3-aequorin
7EG2 2.22 Å, Crystal structure of the apoAequorin complex with (S)-daCTZ

Browse more

1EJ3 is part of these collections:

Fluorescent and glowing proteins

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