1EJ4: Cocrystal structure of EIF4E/4E-BP1 peptide

Name: PDB 1EJ4: Cocrystal structure of EIF4E/4E-BP1 peptide
Published: 2000-03-15
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: translation

Cocrystal structure of EIF4E/4E-BP1 peptide. Determined by X-ray diffraction at 2.25 Å resolution. Released 15 Mar 2000.

Method: X-ray diffraction
Resolution: 2.25 Å
Organism: Mus musculus
Chains: 2
Atoms: 1,713
Mol. weight: 24.46 kDa
Ligands: M7G
Released: 15 Mar 2000

Explore 1EJ4 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1EJ4 contains 12 α-helices and 8 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	38-48	11	1
α-helix	56-59	4
β-strand	60-68	9	1
α-helix	69-78	10
α-helix	80-81	2
α-helix	82-84	3
α-helix	86	1
β-strand	89-95	7	1
β-strand	111-117	7	1
α-helix	119-121	3
α-helix	122-126	5
α-helix	127-138	12
α-helix	143-148	6
β-strand	149-157	9	1
β-strand	160-167	8	1
α-helix	173-187	15
β-strand	196-199	4	1
α-helix	200-204	5
β-strand	215-216	2	1

Chain B: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	56-61	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Eukaryotic initiation factor 4E	A	protein	190	Mus musculus	P63073 (AlphaFold model)
Eukaryotic translation initiation factor 4E binding protein 1	B	protein	14		Q13541 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1EJ4_1 EUKARYOTIC INITIATION FACTOR 4E (chains A)
VANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMP
GCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSD
DVCGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHADTATKS
GSTTKNRFVV

Sequence of entity 2 (B), FASTA

>1EJ4_2 EUKARYOTIC TRANSLATION INITIATION FACTOR 4E BINDING PROTEIN 1 (chains B)
RIIYDRKFLMECRN

Ligands and cofactors

ID	Name	Formula	Copies
M7G	7N-methyl-8-hydroguanosine-5'-diphosphate	C11 H18 N5 O11 P2	1

Primary citation

Cap-dependent translation initiation in eukaryotes is regulated by a molecular mimic of eIF4G. Marcotrigiano, J., Gingras, A.C., Sonenberg, N. et al. Mol Cell (1999) 3:707-716. DOI 10.1016/S1097-2765(01)80003-4 · PubMed

Other PDB entries of the same protein (UniProt P63073 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5M7X 1.68 Å, Translation initiation factor 4E in complex with (RP)-m2(7,2'O)GppSepG mRNA 5' cap…
5M7Z 1.69 Å, Translation initiation factor 4E in complex with (SP)-m2(7,2'O)GppSepG mRNA 5' cap…
5M7V 1.74 Å, Translation initiation factor 4E in complex with (RP)-m2(7,2'O)GppSpG mRNA 5' cap analog…
5J5Y 1.75 Å, Translation initiation factor 4E in complex with m2(7,2'O)GppCCl2ppG mRNA 5' cap analog
6U09 1.79 Å, Discovery of Lysine-Targeted eIF4E Inhibitors through Covalent Docking
1L8B 1.8 Å, Cocrystal Structure of the Messenger RNA 5' Cap-binding Protein (eIF4E) bound to…
5M84 1.85 Å, Translation initiation factor 4E in complex with (SP)-m2(7,2'O)GppSpA mRNA 5' cap analog
6GKK 1.86 Å, Translation initiation factor 4E in complex with beta-phosphorothioate trinucleotide…
5M83 1.86 Å, Translation initiation factor 4E in complex with (RP)-m2(7,2'O)GppSpA mRNA 5' cap analog
5J5O 1.87 Å, Translation initiation factor 4E in complex with m7GppppG mRNA 5' cap analog
5M81 1.9 Å, Translation initiation factor 4E in complex with (SP)-iPr-m7GppSpG mRNA 5' cap analog
5OSX 1.92 Å, Translation initiation factor 4E in complex with m7G(5'S)ppp(5'S)G mRNA 5' cap analog

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