1HUO: DNA polymerase beta

Crystal structure of DNA polymerase beta complexed with DNA and cr-tmppcp. Determined by X-ray diffraction at 2.6 Å resolution. Released 23 Apr 2001.

Method
X-ray diffraction
Resolution
2.6 Å
Organism
Rattus norvegicus
Chains
6
Atoms
5,991
Mol. weight
88.81 kDa
Ligands
CR, TTE
Released
23 Apr 2001

Explore 1HUO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1HUO contains 43 α-helices and 22 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 20 helices, 11 β-strands

ElementResiduesLengthSheet
α-helix13-2412
α-helix25-295
α-helix34-4815
α-helix56-605
α-helix67-7812
α-helix83-908
α-helix92-10110
α-helix108-1169
α-helix122-1254
α-helix134-1418
α-helix1491
β-strand150-15121
α-helix152-16918
β-strand174-17632
α-helix179-1824
β-strand187-18821
β-strand190-19672
α-helix209-22012
β-strand224-23072
β-strand234-23962
α-helix248-2525
β-strand253-25972
α-helix262-2643
α-helix265-2739
α-helix276-28813
β-strand291-29333
β-strand298-30033
β-strand30114
β-strand30714
α-helix310-3123
α-helix316-3227
Chain B: 23 helices, 11 β-strands
ElementResiduesLengthSheet
α-helix13-2816
α-helix33-4816
α-helix56-605
α-helix67-7812
α-helix83-908
α-helix92-1009
α-helix108-1169
α-helix122-1254
α-helix129-1313
α-helix134-14714
α-helix1491
β-strand150-15125
α-helix152-16918
β-strand174-17746
α-helix180-1834
β-strand187-18825
β-strand191-19666
α-helix208-22013
β-strand226-23056
β-strand234-23966
α-helix241-2444
α-helix248-2525
β-strand253-25976
α-helix262-2643
α-helix265-2739
α-helix276-28914
β-strand291-29337
β-strand298-30037
β-strand30118
α-helix3021
β-strand30718
α-helix309-3124
α-helix316-3227
α-helix325-3273

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
5'-d(*ap*ap*tp*ap*gp*gp*cp*gp*tp*cp*g)-3'C, TDNA11
5'-d(p*cp*gp*ap*cp*gp*cp*c)-3'D, PDNA7
DNA polymerase betaA, Bprotein335Rattus norvegicusP06766 (AlphaFold model)
Sequence of entity 1 (C, T), FASTA
>1HUO_1 5'-D(*AP*AP*TP*AP*GP*GP*CP*GP*TP*CP*G)-3' (chains C, T)
AATAGGCGTCG
Sequence of entity 2 (D, P), FASTA
>1HUO_2 5'-D(P*CP*GP*AP*CP*GP*CP*C)-3' (chains D, P)
CGACGCC
Sequence of entity 3 (A, B), FASTA
>1HUO_3 DNA POLYMERASE BETA (chains A, B)
MSKRKAPQETLNGGITDMLVELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAK
KLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVTGIGPSAARKLVDEGIK
TLEDLRKNEDKLNHHQRIGLKYFEDFEKRIPREEMLQMQDIVLNEVKKLDPEYIATVCGS
FRRGAESSGDMDVLLTHPNFTSESSKQPKLLHRVVEQLQKVRFITDTLSKGETKFMGVCQ
LPSENDENEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRP
LGVTGVAGEPLPVDSEQDIFDYIQWRYREPKDRSE

Ligands and cofactors

IDNameFormulaCopies
CRChromium ionCr2
TTEPhosphomethyl phosphonic acid deoxythymidylate esterC11 H19 N2 O13 P32

Primary citation

Insight into the catalytic mechanism of DNA polymerase beta: structures of intermediate complexes. Arndt, J.W., Gong, W., Zhong, X. et al. Biochemistry (2001) 40:5368-5375. DOI 10.1021/bi002176j · PubMed

Other PDB entries of the same protein (UniProt P06766 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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