1MHP: Chimeric alpha1 integrin I-domain

Crystal structure of a chimeric alpha1 integrin I-domain in complex with the Fab fragment of a humanized neutralizing antibody. Determined by X-ray diffraction at 2.8 Å resolution. Released 15 Apr 2003.

Method: X-ray diffraction
Resolution: 2.8 Å
Organisms: Rattus norvegicus, Mus musculus
Chains: 6
Atoms: 7,943
Mol. weight: 136.06 kDa
Ligands: MN
Released: 15 Apr 2003

Explore 1MHP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1MHP contains 42 α-helices and 87 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	147-154	8	1
α-helix	162-174	13
β-strand	178	1	2
β-strand	183	1	2
β-strand	184-190	7	1
β-strand	194-198	5	1
α-helix	206-214	9
α-helix	216-218	3
α-helix	226-232	7
α-helix	233-237	5
α-helix	240-242	3
β-strand	249-256	8	1
α-helix	266-275	10
β-strand	278-285	8	1
α-helix	297-304	8
α-helix	308	1
α-helix	311-313	3
β-strand	315-318	4	1
α-helix	321-324	4
α-helix	325-334	10

Chain B: 13 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	147-154	8	13
α-helix	162-174	13
β-strand	178	1	14
β-strand	183	1	14
β-strand	184-190	7	13
β-strand	194-198	5	13
α-helix	206-214	9
α-helix	216-218	3
α-helix	226-232	7
α-helix	233-237	5
α-helix	240-242	3
β-strand	249-256	8	13
α-helix	266-275	10
β-strand	278-285	8	13
α-helix	287-291	5
α-helix	297-304	8
α-helix	308	1
α-helix	311-314	4
β-strand	315-318	4	13
α-helix	321-327	7
α-helix	328-334	7

Chain H: 10 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	3
β-strand	10-12	3	4
β-strand	18-25	8	3
α-helix	29-31	3
α-helix	33	1
β-strand	34-39	6	4
β-strand	45-51	7	4
β-strand	57-59	3	4
α-helix	61-63	3
β-strand	64	1	3
β-strand	67-72	6	3
β-strand	77-82	6	3
α-helix	87-89	3
β-strand	91-99	9	4
β-strand	104-108	5	4
β-strand	109	1	3
β-strand	112-116	5	4
α-helix	119-121	3
β-strand	122	1	5
α-helix	123-124	2
β-strand	125-129	5	6
β-strand	136-137	2	6
β-strand	140-150	11	6
β-strand	151	1	5
β-strand	156-159	4	7
α-helix	160-162	3
β-strand	164	1	7
β-strand	168-170	3	6
α-helix	171-173	3
β-strand	174-175	2	6
β-strand	181-190	10	6
α-helix	191-194	4
β-strand	199-205	7	7
α-helix	206-208	3
β-strand	210-216	7	7

Chain L: 4 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	8
β-strand	10-13	4	9
β-strand	19-25	7	8
β-strand	33-37	5	9
β-strand	44-48	5	9
β-strand	52-53	2	9
β-strand	61-66	6	8
β-strand	69-74	6	8
β-strand	84-89	6	9
β-strand	96-97	2	9
β-strand	101-105	5	9
β-strand	110	1	10
α-helix	111-112	2
β-strand	113-117	5	11
α-helix	118-120	3
α-helix	121-124	4
β-strand	128-138	11	11
β-strand	139	1	10
β-strand	143-149	7	12
β-strand	152-153	2	12
β-strand	158-162	5	11
β-strand	172-181	10	11
α-helix	182-186	5
β-strand	190-197	8	12
β-strand	204-209	6	12

Chain X: 3 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	15
β-strand	11-12	2	16
β-strand	18-25	8	15
α-helix	29-31	3
α-helix	33	1
β-strand	34-39	6	17
β-strand	45-51	7	17
β-strand	57-59	3	17
β-strand	64	1	18
β-strand	67	1	18
β-strand	68-72	5	15
α-helix	73-75	3
β-strand	77-82	6	15
β-strand	91-99	9	17
β-strand	104-108	5	17
β-strand	112-114	3	17
β-strand	115-116	2	16

Chain Y: 0 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	19
β-strand	10-13	4	20
β-strand	19-25	7	19
β-strand	33-37	5	20
β-strand	44-48	5	20
β-strand	52-53	2	20
β-strand	61-66	6	19
β-strand	69-74	6	19
β-strand	84-89	6	20
β-strand	96-97	2	20
β-strand	101-105	5	20

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
integrin alpha 1, (RESIDUES 169-360)	A, B	protein	192	Rattus norvegicus	P18614 (AlphaFold model)
Fab fragment, heavy chain	H, X	protein	219	Mus musculus
FAB FRAGMENT, light chain	L, Y	protein	212	Mus musculus

Sequence of entity 1 (A, B), FASTA

>1MHP_1 integrin alpha 1, (RESIDUES 169-360) (chains A, B)
TQLDIVIVLDGSNSIYPWESVIAFLNDLLKRMDIGPKQTQVGIVQYGENVTHEFNLNKYS
STEEVLVAANKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNY
RLKQVIQDCEDENIQRFSIAILGHYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELAL
VTIVKALGERIF

Sequence of entity 2 (H, X), FASTA

>1MHP_2 Fab fragment, heavy chain (chains H, X)
EVQLVESGGGLVQPGGSLRLSCAASGFTFSRYTMSWVRQAPGKGLEWVATISGGGHTYYL
DSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCTRGFGDGGYFDVWGQGTLVTVSSAS
TKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGL
YSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK

Sequence of entity 3 (L, Y), FASTA

>1MHP_3 FAB FRAGMENT, light chain (chains L, Y)
IQLTQSPSSLSASVGDRVTITCSASSSVNHMFWYQQKPGKAPKPWIYLTSNLASGVPSRF
SGSGSGTDYTLTISSLQPEDFATYYCQQWSGNPWTFGQGTKVEIKRTVAAPSVFIFPPSD
EQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLS
KADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Ligands and cofactors

ID	Name	Formula	Copies
MN	Manganese (II) ion	Mn	2

Primary citation

Crystal Structure of the alpha 1 beta 1 Integrin I Domain in Complex with an Antibody Fab Fragment. Karpusas, M., Ferrant, J., Weinreb, P. et al. J Mol Biol (2003) 327:1031-1041. DOI 10.1016/S0022-2836(03)00203-1 · PubMed

Other PDB entries of the same protein (UniProt P18614 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1CK4 2.2 Å, Crystal structure of rat A1B1 integrin I-domain.
2B2X 2.2 Å, VLA1 RdeltaH I-domain complexed with a quadruple mutant of the AQC2 Fab

Browse structure collections

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