2F8X: PDB entry 2F8X

Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence. Determined by X-ray diffraction at 3.25 Å resolution. Released 4 Apr 2006.

Method
X-ray diffraction
Resolution
3.25 Å
Organism
Homo sapiens
Chains
5
Atoms
6,236
Mol. weight
95.74 kDa
Released
4 Apr 2006

Explore 2F8X in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2F8X contains 28 α-helices and 51 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain C: 12 helices, 51 β-strands

ElementResiduesLengthSheet
α-helix12-143
α-helix16-183
α-helix19-279
β-strand32-3321
β-strand36-3942
β-strand41-4333
β-strand4514
β-strand58-5922
β-strand6211
α-helix65-7410
β-strand88-9145
α-helix981
β-strand99-10025
β-strand11012
β-strand11714
β-strand124-12523
β-strand127-13265
β-strand138-14365
α-helix144-1463
β-strand147-15043
β-strand16616
β-strand16817
β-strand172-17762
α-helix179-1813
β-strand187-18822
β-strand190-19238
β-strand195-19738
β-strand206-21162
β-strand22717
β-strand22919
β-strand233-23862
β-strand24412
β-strand248-25252
β-strand253-254210
β-strand257-259310
β-strand26519
β-strand26716
β-strand270-27562
β-strand28312
β-strand285-286211
β-strand291-292211
β-strand300112
β-strand302112
β-strand305-307310
β-strand314-32292
β-strand325-32621
β-strand329113
β-strand331113
α-helix3381
α-helix3401
β-strand342-348714
α-helix352-3543
β-strand356-362714
β-strand368-372515
β-strand375-377315
β-strand379-383514
β-strand386-390514
α-helix391-3933
α-helix394-3974
β-strand408-409216
β-strand412-416515
β-strand421-423315
β-strand429-430216
Chain K: 15 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix1885-18917
α-helix1910-19156
α-helix1923-19253
α-helix1932-19387
α-helix1942-19509
α-helix1965-19717
α-helix1975-19839
α-helix1999-20057
α-helix2011-20177
α-helix2032-20387
α-helix2042-20509
α-helix2065-20728
α-helix2075-20839
α-helix2098-21047
α-helix2108-21169
Chain M: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix17-6953

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
5'-d(*gp*tp*tp*ap*cp*tp*gp*tp*gp*gp*gp*ap*ap*ap*gp*ap*ap*a)-3'XDNA18
5'-d(*tp*tp*tp*cp*tp*tp*tp*cp*cp*cp*ap*cp*ap*gp*tp*ap*ap*c)-3'YDNA18
Recombining binding protein suppressor of hairless, isoform 4Cprotein434Homo sapiensQ06330 (AlphaFold model)
Neurogenic locus notch homolog protein 1Kprotein256Homo sapiensP46531 (AlphaFold model)
Mastermind-like protein 1Mprotein63Homo sapiensQ92585 (AlphaFold model)
Sequence of entity 1 (X), FASTA
>2F8X_1 5'-D(*GP*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*AP*AP*A)-3' (chains X)
GTTACTGTGGGAAAGAAA
Sequence of entity 2 (Y), FASTA
>2F8X_2 5'-D(*TP*TP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*TP*AP*AP*C)-3' (chains Y)
TTTCTTTCCCACAGTAAC
Sequence of entity 3 (C), FASTA
>2F8X_3 Recombining binding protein suppressor of hairless, isoform 4 (chains C)
MGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNEKRFFCPPPCVYLMGSGWK
KKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFML
SVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTR
YLHVEGGNFHASSQQWGAFFIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPR
LIIRKVDKQTALLDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEPNKEM
INDGASWTIISTDKAEYTFYEGMGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPN
LRVWFGDVEAETMYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLT
FTYTPEPGHHHHHH
Sequence of entity 4 (K), FASTA
>2F8X_4 Neurogenic locus notch homolog protein 1 (chains K)
GMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGET
ALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDA
RMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKN
GANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIV
RLLDEYNLVRSPQLHG
Sequence of entity 5 (M), FASTA
>2F8X_5 Mastermind-like protein 1 (chains M)
GLPRHSAVMERLRRRIELCRRHHSTCEARYEAVSPERLELERQHTFALHQRCIQAKAKRA
GKH

Primary citation

Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes. Nam, Y., Sliz, P., Song, L. et al. Cell (2006) 124:973-983. DOI 10.1016/j.cell.2005.12.037 · PubMed

Other PDB entries of the same protein (UniProt Q06330 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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