2FE8: Replicase polyprotein 1ab

SARS coronavirus papain-like protease: structure of a viral deubiquitinating enzyme. Determined by X-ray diffraction at 1.85 Å resolution. Released 21 Mar 2006.

Method: X-ray diffraction
Resolution: 1.85 Å
Organism: SARS coronavirus
Chains: 3
Atoms: 7,978
Mol. weight: 107.38 kDa
Ligands: ZN
Released: 21 Mar 2006

Explore 2FE8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2FE8 contains 33 α-helices and 63 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	5-11	7	1
β-strand	18-23	6	1
α-helix	28-32	5
β-strand	35-37	3	1
β-strand	40-41	2	1
α-helix	45-48	4
α-helix	49-51	3
β-strand	55-58	4	1
α-helix	63-73	11
α-helix	80-91	12
β-strand	98-99	2	2
β-strand	102-103	2	2
α-helix	112-122	11
β-strand	128	1	3
α-helix	131-142	12
α-helix	146-155	10
α-helix	166-174	9
β-strand	177	1	3
β-strand	183-190	8	4
β-strand	194-201	8	4
α-helix	203-206	4
β-strand	207-209	3	5
α-helix	214-219	6
β-strand	221-224	4	4
β-strand	230-239	10	4
β-strand	242-255	14	5
β-strand	261-266	6	5
α-helix	269-271	3
β-strand	273-279	7	5
β-strand	283-287	5	5
β-strand	290-294	5	5
β-strand	296-307	12	5
β-strand	310-312	3	4

Chain B: 11 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	5-11	7	6
β-strand	18-23	6	6
α-helix	28-32	5
β-strand	35-37	3	6
β-strand	40-41	2	6
α-helix	46-48	3
α-helix	49-51	3
β-strand	55-58	4	6
α-helix	63-73	11
α-helix	80-91	12
β-strand	98-99	2	7
β-strand	102-103	2	7
α-helix	112-121	10
β-strand	128	1	8
α-helix	131-141	11
α-helix	146-156	11
α-helix	166-174	9
β-strand	177	1	8
β-strand	183-190	8	9
β-strand	194-201	8	9
α-helix	203-206	4
β-strand	207-209	3	10
α-helix	214-219	6
β-strand	221-224	4	9
β-strand	230-239	10	9
β-strand	242-255	14	10
β-strand	261-266	6	10
β-strand	273-279	7	10
β-strand	283-287	5	10
β-strand	290-294	5	10
β-strand	296-307	12	10
β-strand	310-312	3	9

Chain C: 10 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	5-11	7	11
β-strand	18-23	6	11
α-helix	28-32	5
β-strand	35-37	3	11
β-strand	40-41	2	11
α-helix	49-51	3
β-strand	55-58	4	11
α-helix	63-73	11
α-helix	80-91	12
β-strand	98-99	2	12
β-strand	102-103	2	12
α-helix	112-121	10
β-strand	128	1	13
α-helix	131-142	12
α-helix	146-155	10
α-helix	166-174	9
β-strand	177	1	13
β-strand	183-190	8	14
β-strand	194-201	8	14
α-helix	203-206	4
β-strand	207-209	3	15
α-helix	214-219	6
β-strand	221-224	4	14
β-strand	230-239	10	14
β-strand	242-255	14	15
β-strand	261-266	6	15
β-strand	273-279	7	15
β-strand	283-287	5	15
β-strand	290-294	5	15
β-strand	296-307	12	15
β-strand	310-312	3	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Replicase polyprotein 1ab	A, B, C	protein	315	SARS coronavirus	P0C6X7 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>2FE8_1 Replicase polyprotein 1ab (chains A, B, C)
MEVKTIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLP
SDDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVLLA
LQQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLE
SAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQES
SFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGP
VTDVFYKETSYTTTI

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	3

Water and common crystallization additives (BR, SO4) are not listed.

Primary citation

Severe acute respiratory syndrome coronavirus papain-like protease: Structure of a viral deubiquitinating enzyme. Ratia, K., Saikatendu, K.S., Santarsiero, B.D. et al. Proc Natl Acad Sci U S A (2006) 103:5717-5722. DOI 10.1073/pnas.0510851103 · PubMed

Other PDB entries of the same protein (UniProt P0C6X7 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2ACF 1.4 Å, NMR structure of SARS-CoV non-structural protein NSP3A (SARS1) from SARS coronavirus
6W79 1.46 Å, Structure of SARS-CoV main protease bound to potent broad-spectrum non-covalent…
6WCO 1.48 Å, Structure of SARS main protease bound to inhibitor X47
5C5O 1.5 Å, Structure of SARS-3CL protease complex with a phenyl-beta-alanyl (S,R)-N-decalin type…
8CB3 1.57 Å, SARS-CoV Macro domain complexed with 3-(N-morpholino)propanesulfonic acid
3TNT 1.59 Å, Crystal structure of SARS coronavirus main protease complexed with an alpha,…
4TWY 1.6 Å, Structure of SARS-3CL protease complex with a phenylbenzoyl (S,R)-N-decalin type inhibitor
7FA1 1.6 Å, Crystal Structure of N-terminus of the non-structural protein 2 from SARS coronavirus
5N19 1.62 Å, Structure of SARS coronavirus main protease in complex with the alpha-ketoamide…
2ZU5 1.65 Å, complex structure of SARS-CoV 3CL protease with TG-0205486
8UFM 1.65 Å, Crystal Structure of L516C/Y647C Mutant of SARS-Unique Domain (SUD) from SARS-CoV-2
3SZN 1.69 Å, Crystal structure of SARS coronavirus main protease complexed with an alpha,…

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