2HFG: HBR3

Crystal structure of hBR3 bound to CB3s-Fab. Determined by X-ray diffraction at 2.61 Å resolution. Released 7 Nov 2006.

Method: X-ray diffraction
Resolution: 2.61 Å
Organism: Homo sapiens
Chains: 3
Atoms: 3,475
Mol. weight: 52.99 kDa
Released: 7 Nov 2006

Explore 2HFG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2HFG contains 17 α-helices and 47 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain H: 7 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	6
β-strand	10-12	3	7
β-strand	18-25	8	6
α-helix	29-31	3
β-strand	33-39	7	7
β-strand	45-52	8	7
β-strand	56-59	4	7
β-strand	64	1	6
β-strand	67-72	6	6
α-helix	73-75	3
β-strand	77-82	6	6
α-helix	84-86	3
β-strand	88-100	13	7
β-strand	100C-103	9	7
β-strand	107-111	5	7
α-helix	115-116	2
β-strand	117	1	8
β-strand	120-124	5	9
β-strand	135-145	11	9
β-strand	146	1	8
β-strand	151-154	4	10
α-helix	155-157	3
β-strand	159	1	10
β-strand	163-165	3	9
α-helix	166-168	3
β-strand	169-170	2	9
β-strand	176-185	10	9
β-strand	195-200	6	10
α-helix	201-203	3
β-strand	205-210	6	10

Chain L: 9 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	1
β-strand	10-14	5	2
β-strand	19-25	7	1
β-strand	33-38	6	2
β-strand	45-49	5	2
β-strand	53-54	2	2
α-helix	55	1
β-strand	62-67	6	1
β-strand	70-75	6	1
α-helix	80-82	3
β-strand	84-90	7	2
α-helix	97	1
β-strand	98	1	2
α-helix	99	1
β-strand	102-106A	6	2
β-strand	110	1	3
α-helix	111-112	2
β-strand	113-117	5	4
α-helix	118-120	3
α-helix	121-126	6
β-strand	128-138	11	4
β-strand	139	1	3
β-strand	144-149	6	5
β-strand	152-153	2	5
β-strand	158-162	5	4
α-helix	163-166	4
β-strand	172-181	10	4
α-helix	182-186	5
β-strand	190-196	7	5
β-strand	204-209	6	5

Chain R: 1 helix, 3 β-strands

Element	Residues	Length	Sheet
α-helix	14-20	7
β-strand	23-26	4	7
β-strand	31-34	4	7
β-strand	36-37	2	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
CB3s Fab light chain (kappa)	L	protein	214	Homo sapiens	Q6PIH7
CB3s Fab heavy chain	H	protein	232	Homo sapiens	Q6N093 (AlphaFold model)
Tumor necrosis factor receptor superfamily member 13C	R	protein	51	Homo sapiens	Q96RJ3 (AlphaFold model)

Sequence of entity 1 (L), FASTA

>2HFG_1 CB3s Fab light chain (kappa) (chains L)
DIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPS
RFSGSGSGTDFTLTISSLQPEDFATYYCQQSQISPPTFGQGTKVEIKRTVAAPSVFIFPP
SDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLT
LSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Sequence of entity 2 (H), FASTA

>2HFG_2 CB3s Fab heavy chain (chains H)
EVQLVESGGGLVQPGGSLRLSCAASGFTISSSSIHWVRQAPGKGLEWVAWVLPSVGFTDY
ADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARRVCYNRLGVCAGGMDYWGQGTL
VTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPA
VLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH

Sequence of entity 3 (R), FASTA

>2HFG_3 Tumor necrosis factor receptor superfamily member 13C (chains R)
GSYSLRGRDAPAPTPCNPAECFDPLVRHCVACGLLRTPRPKPAGASSPAPR

Primary citation

Synthetic anti-BR3 antibodies that mimic BAFF binding and target both human and murine B cells. Lee, C.V., Hymowitz, S.G., Wallweber, H.J. et al. Blood (2006) 108:3103-3111. DOI 10.1182/blood-2006-03-011031 · PubMed

Other PDB entries of the same protein (UniProt Q6PIH7), best resolution first:

2AJ3 2.03 Å, Crystal Structure of a Cross-Reactive HIV-1 Neutralizing CD4-Binding Site Antibody Fab m18

Browse structure collections

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