2K5X: Colicin-E9 immunity protein

Chemical shift structure of COLICIN E9 DNASE domain with its cognate immunity protein IM9. Determined by solution NMR. Released 9 Dec 2008.

Method: Solution NMR
Organism: Escherichia coli
Chains: 2
Atoms: 1,737
Mol. weight: 24.71 kDa
Released: 9 Dec 2008

Explore 2K5X in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2K5X contains 14 α-helices and 15 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	7-9	3
α-helix	12-23	12
α-helix	30-44	15
α-helix	50-54	5
α-helix	56-57	2
β-strand	58	1	1
β-strand	60	1	1
α-helix	64-78	15

Chain B: 8 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	9-10	2	2
β-strand	12	1	3
β-strand	16	1	4
α-helix	22-25	4
β-strand	32-33	2	5
α-helix	34	1
β-strand	35	1	4
α-helix	36-40	5
β-strand	46-47	2	2
α-helix	50-63	14
α-helix	73-80	8
β-strand	86	1	6
β-strand	93	1	7
β-strand	96	1	7
β-strand	98	1	6
β-strand	100-103	4	5
α-helix	107-109	3
β-strand	115	1	3
α-helix	116-118	3
β-strand	119-122	4	5
α-helix	124-130	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Colicin-E9 immunity protein	A	protein	86	Escherichia coli	P13479 (AlphaFold model)
Colicin-E9	B	protein	134	Escherichia coli	P09883 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2K5X_1 Colicin-E9 immunity protein (chains A)
MELKHSISDYTEAEFLQLVTTICNADTSSEEELVKLVTHFEEMTEHPSGSDLIYYPKEGD
DDSPSGIVNTVKQWRAANGKSGFKQG

Sequence of entity 2 (B), FASTA

>2K5X_2 Colicin-E9 (chains B)
MESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKAVWEE
VSKDPELSKNLNPSNKSSVSKGYSPFTPKNQQVGGRKVYELHHDKPISQGGEVYDMDNIR
VTTPKRHIDIHRGK

Primary citation

Structure Determination of Protein-Protein Complexes Using NMR Chemical Shifts: Case of an Endonuclease Colicin-Immunity Protein Complex. Montalvao, R.W., Cavalli, A., Salvatella, X. et al. J Am Chem Soc (2008) 130:15990-15996. PubMed

Other PDB entries of the same protein (UniProt P13479 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1FR2 1.6 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9(E41A)
2GYK 1.6 Å, Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity…
2GZJ 1.6 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (D51A)
2VLN 1.6 Å, N75A mutant of E9 DNase domain in complex with Im9
2VLQ 1.6 Å, F86A mutant of E9 DNase domain in complex with Im9
1EMV 1.7 Å, Crystal structure of colicin E9 DNase domain with its cognate immunity protein IM9 (1.7 Å)
2GZG 1.7 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (Y55F)
2GZI 1.7 Å, Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (V34A)
2GZF 1.75 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y54F)
2GZE 1.8 Å, Crystal structure of the E9 DNase domain with a mutant immunity protein IM9 (Y55A)
2VLO 1.8 Å, K97A mutant of E9 DNase domain in complex with Im9
2VLP 2.0 Å, R54A mutant of E9 DNase domain in complex with Im9

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