2O25: Ubiquitin-Conjugating Enzyme E2-25 kDa

Ubiquitin-Conjugating Enzyme E2-25 kDa Complexed With SUMO-1-Conjugating Enzyme UBC9. Determined by X-ray diffraction at 2.6 Å resolution. Released 16 Jan 2007.

Method: X-ray diffraction
Resolution: 2.6 Å
Organism: Homo sapiens
Chains: 4
Atoms: 5,687
Mol. weight: 81.5 kDa
Released: 16 Jan 2007

Explore 2O25 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2O25 contains 41 α-helices and 29 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	3-17	15
α-helix	20-23	4
β-strand	27-31	5	1
β-strand	38-44	7	1
α-helix	45-46	2
β-strand	55-61	7	1
α-helix	70-71	2
β-strand	72-75	4	1
β-strand	81	1	2
β-strand	84	1	2
β-strand	90	1	1
β-strand	91	1	2
α-helix	94-96	3
α-helix	106-116	11
α-helix	128-136	9
α-helix	138-153	16
α-helix	155	1
α-helix	157	1
α-helix	160-171	12
α-helix	176-185	10
α-helix	190-198	9

Chain B: 12 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	7-11	5
α-helix	13-18	6
β-strand	27-31	5	3
β-strand	38-44	7	3
α-helix	45-46	2
β-strand	55-61	7	3
α-helix	70-71	2
β-strand	72-75	4	3
β-strand	84	1	4
β-strand	90	1	3
β-strand	91	1	4
α-helix	94-96	3
α-helix	106-118	13
α-helix	128-136	9
α-helix	138-152	15
α-helix	155-157	3
α-helix	160-171	12
α-helix	176-185	10
α-helix	190-197	8

Chain C: 8 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	4-18	15
β-strand	25-30	6	5
β-strand	36-46	11	5
α-helix	47-48	2
β-strand	57-63	7	5
α-helix	72-73	2
β-strand	74-77	4	5
α-helix	80-81	2
β-strand	86	1	6
β-strand	91	1	5
β-strand	92	1	6
α-helix	95-97	3
α-helix	109-121	13
α-helix	131-139	9
α-helix	141-154	14

Chain D: 8 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	3-18	16
β-strand	25-28	4	7
α-helix	29-31	3
β-strand	40-46	7	7
α-helix	47-48	2
β-strand	57-63	7	7
α-helix	72-73	2
β-strand	74-77	4	7
β-strand	86	1	8
β-strand	91	1	7
β-strand	92	1	8
α-helix	95-97	3
α-helix	109-121	13
α-helix	131-137	7
α-helix	141-153	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Ubiquitin-conjugating enzyme E2-25 kDa	A, B	protein	202	Homo sapiens	P61086 (AlphaFold model)
SUMO-1-conjugating enzyme UBC9	C, D	protein	160	Homo sapiens	P63279 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>2O25_1 Ubiquitin-conjugating enzyme E2-25 kDa (chains A, B)
GSMANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQL
EIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLA
AAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNA
VIVALSSKSWDVETATELLLSN

Sequence of entity 2 (C, D), FASTA

>2O25_2 SUMO-1-conjugating enzyme UBC9 (chains C, D)
GSMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLF
KLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQE
LLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Primary citation

A Novel and Unexpected Complex Between the SUMO-1-Conjugating Enzyme UBC9 and the Ubiquitin-Conjugating Enzyme E2-25 kDa. Walker, J.R., Avvakumov, G.V., Xue, S. et al. To be published.

Other PDB entries of the same protein (UniProt P61086 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3K9O 1.8 Å, The crystal structure of E2-25K and UBB+1 complex
3E46 1.86 Å, Crystal structure of ubiquitin-conjugating enzyme E2-25kDa (Huntington interacting…
5DFL 2.1 Å, Crystal structure of Ube2K~Ubiquitin conjugate
6JB7 2.1 Å, Crystal structure of Ub-conjugated Ube2K C92K&K97A mutant (isopeptide linkage), 2.1 A…
3F92 2.23 Å, Crystal structure of ubiquitin-conjugating enzyme E2-25kDa (Huntington Interacting…
7MYH 2.39 Å, Ubiquitin variant UbV.k.2 in complex with Ube2k
1YLA 2.4 Å, Ubiquitin-conjugating enzyme E2-25 kDa (Huntington interacting protein 2)
7OJX 2.4 Å, E2 UBE2K covalently linked to donor Ub, acceptor di-Ub, and RING E3 primed for…
6IF1 2.47 Å, Crystal structure of Ube2K and K48-linked di-ubiquitin complex
6JB6 2.7 Å, Crystal structure of Ub-conjugated Ube2K C92K&K97A mutant (isopeptide linkage), 2.7 A…
3K9P 2.8 Å, The crystal structure of E2-25K and ubiquitin complex
7MYF 3.0 Å, Ubiquitin variant UbV.k.1 in complex with Ube2k

Browse structure collections

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