2Q7T: Protein traI

Crystal Structure of the F plasmid TraI Relaxase Domain with the Scissile Thymidine Base. Determined by X-ray diffraction at 2.42 Å resolution. Released 20 May 2008.

Method: X-ray diffraction
Resolution: 2.42 Å
Organism: Escherichia coli
Chains: 2
Atoms: 4,321
Mol. weight: 67.08 kDa
Ligands: TMP, MG
Released: 20 May 2008

Explore 2Q7T in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2Q7T contains 28 α-helices and 28 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	1
α-helix	10-17	8
α-helix	20-22	3
β-strand	32-34	3	1
α-helix	36-41	6
α-helix	49-56	8
β-strand	59	1	2
β-strand	65	1	2
β-strand	69-70	2	3
β-strand	73-74	2	3
β-strand	79-85	7	1
α-helix	88-95	8
α-helix	101-118	18
β-strand	122-124	3	4
β-strand	133-135	3	4
β-strand	141-148	8	1
β-strand	154-163	10	1
β-strand	166-168	3	5
β-strand	171-173	3	5
α-helix	174-176	3
α-helix	185-190	6
α-helix	193-210	18
β-strand	216-217	2	6
α-helix	220-222	3
β-strand	224-225	2	6
α-helix	231-234	4
α-helix	270-282	13
α-helix	288-296	9

Chain B: 15 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	1002-1007	6	7
α-helix	1010-1017	8
α-helix	1020-1022	3
β-strand	1032-1034	3	7
α-helix	1036-1040	5
α-helix	1047-1048	2
α-helix	1049-1056	8
β-strand	1079-1085	7	7
α-helix	1088-1091	4
α-helix	1092-1096	5
α-helix	1101-1118	18
β-strand	1122-1124	3	8
β-strand	1127	1	9
β-strand	1130	1	9
β-strand	1133-1135	3	8
β-strand	1141-1148	8	7
β-strand	1154-1163	10	7
β-strand	1166-1168	3	10
β-strand	1171-1173	3	10
α-helix	1174-1176	3
α-helix	1185-1191	7
α-helix	1193-1210	18
β-strand	1216-1217	2	11
α-helix	1220-1222	3
β-strand	1224-1225	2	11
α-helix	1231-1234	4
α-helix	1270-1282	13
α-helix	1288-1296	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein traI	A, B	protein	301	Escherichia coli	P14565 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>2Q7T_1 Protein traI (chains A, B)
MMSIAQVRSAGSAGNFYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRL
PDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV
GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT
IREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQTLKETGFDIRAYRDAADQRAD
S

Ligands and cofactors

ID	Name	Formula	Copies
TMP	Thymidine-5'-phosphate	C10 H15 N2 O8 P	1
MG	Magnesium ion	Mg	2

Primary citation

Disrupting antibiotic resistance propagation by inhibiting the conjugative DNA relaxase. Lujan, S.A., Guogas, L.M., Ragonese, H. et al. Proc Natl Acad Sci U S A (2007) 104:12282-12287. DOI 10.1073/pnas.0702760104 · PubMed

Other PDB entries of the same protein (UniProt P14565 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3FLD 2.4 Å, Crystal structure of the trai c-terminal domain
1P4D 2.6 Å, F factor TraI Relaxase Domain
2A0I 2.72 Å, F Factor TraI Relaxase Domain bound to F oriT Single-stranded DNA
9F10 2.94 Å, CryoEM structure of the F plasmid relaxosome with TraI in its TE mode, without accessory…
2Q7U 3.0 Å, Crystal Structure of the F plasmid TraI Relaxase Domain with the Scissile Thymidine Base…
9F0Z 3.42 Å, CryoEM structure of the F plasmid relaxosome with truncated TraI1-863 in its TE mode,…
9F12 3.42 Å, CryoEM structure of the F plasmid relaxosome with oriT DNA ss-27_-3ds-2_+143 and TraI…
9F0Y 3.45 Å, CryoEM structure of the F plasmid relaxosome with TraI in its TE mode, derived from the…
9F11 3.68 Å, CryoEM structure of the F plasmid relaxosome with oriT DNA ss-27_+3ds+4_+143 and TraI…
9F0X 3.78 Å, CryoEM structure of the F plasmid relaxosome in its pre-initiation state, derived from…
2L8B TraI (381-569)

Browse structure collections

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