2QCS: PDB entry 2QCS

A complex structure between the Catalytic and Regulatory subunit of Protein Kinase A that represents the inhibited state. Determined by X-ray diffraction at 2.2 Å resolution. Released 6 Nov 2007.

Method: X-ray diffraction
Resolution: 2.2 Å
Organisms: Mus musculus, Bos taurus
Chains: 2
Atoms: 5,459
Mol. weight: 75.15 kDa
Ligands: MN, ANP, TAM
Released: 6 Nov 2007

Explore 2QCS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2QCS contains 31 α-helices and 31 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	14-31	18
α-helix	40-42	3
β-strand	43-51	9	1
β-strand	56-62	7	1
β-strand	68-75	8	1
α-helix	76-81	6
α-helix	85-97	13
β-strand	103	1	2
α-helix	104-105	2
β-strand	106-111	6	1
β-strand	115-121	7	1
α-helix	122	1
β-strand	127	1	2
α-helix	128-135	8
α-helix	140-159	20
β-strand	162-163	2	3
α-helix	169-171	3
β-strand	172-174	3	2
β-strand	180-182	3	2
β-strand	189-190	2	3
β-strand	195	1	4
β-strand	199-200	2	5
α-helix	202-204	3
α-helix	207-210	4
β-strand	215	1	4
α-helix	219-233	15
α-helix	243-252	10
α-helix	263-272	10
α-helix	289-292	4
α-helix	295-297	3
α-helix	302-306	5

Chain B: 14 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	94-97	4
β-strand	98-99	2	5
α-helix	105-109	5
α-helix	120-129	10
α-helix	134-137	4
α-helix	141-150	10
β-strand	152-156	5	6
β-strand	161-163	3	7
β-strand	168	1	8
α-helix	169	1
β-strand	171-177	7	6
β-strand	180-184	5	7
β-strand	187-192	6	7
β-strand	197-198	2	6
α-helix	200-203	4
β-strand	208	1	8
β-strand	212-215	4	7
β-strand	219-225	7	6
α-helix	226-249	24
α-helix	252-254	3
α-helix	259-268	10
β-strand	270-274	5	9
β-strand	279-281	3	9
β-strand	289-303	15	9
β-strand	310-316	7	9
β-strand	321-322	2	9
α-helix	325-327	3
β-strand	336-349	14	9
α-helix	350-357	8
α-helix	360-364	5
α-helix	368-374	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
cAMP-dependent protein kinase, alpha-catalytic subunit	A	protein	350	Mus musculus	P05132 (AlphaFold model)
cAMP-dependent protein kinase type I-alpha regulatory subunit	B	protein	291	Bos taurus	P00514 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2QCS_1 cAMP-dependent protein kinase, alpha-catalytic subunit (chains A)
GNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLV
KHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVM
EYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYI
QVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFA
DQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATT
DWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF

Sequence of entity 2 (B), FASTA

>2QCS_2 cAMP-dependent protein kinase type I-alpha regulatory subunit (chains B)
KGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFD
AMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPR
AATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADAL
EPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLM
NRPKAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSVA

Ligands and cofactors

ID	Name	Formula	Copies
MN	Manganese (II) ion	Mn	2
ANP	Phosphoaminophosphonic acid-adenylate ester	C10 H17 N6 O12 P3	1
TAM	Tris(hydroxyethyl)aminomethane	C7 H17 N O3	1

Water and common crystallization additives (SO4, ACT, GOL) are not listed.

Primary citation

PKA-I holoenzyme structure reveals a mechanism for cAMP-dependent activation. Kim, C., Cheng, C.Y., Saldanha, S.A. et al. Cell (2007) 130:1032-1043. DOI 10.1016/j.cell.2007.07.018 · PubMed

Other PDB entries of the same protein (UniProt P05132 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1RDQ 1.26 Å, Hydrolysis of ATP in the crystal of Y204A mutant of cAMP-dependent protein kinase
4DFX 1.35 Å, Crystal structure of myristoylated K7C catalytic subunit of cAMP-dependent protein…
4HPU 1.55 Å, Crystal structure of the catalytic subunit of cAMP-dependent protein kinase displaying…
4IAI 1.55 Å, X-ray Structure of cAMP dependent protein kinase A in complex with high Ca2+…
9PC1 1.55 Å, Co-crystal structure of the cAMP-dependent protein kinase catalytic subunit alpha with…
3FJQ 1.6 Å, Crystal structure of cAMP-dependent protein kinase catalytic subunit alpha in complex…
4IAK 1.6 Å, Low temperature X-ray structure of cAMP dependent protein kinase A in complex with high…
3IDB 1.62 Å, Crystal structure of (108-268)RIIb:C holoenzyme of cAMP-dependent protein kinase
4IB1 1.63 Å, Structure of cAMP dependent protein kinase A in complex with high K+ concentration, ADP…
7V0G 1.63 Å, Structure of cAMP-dependent protein kinase using a MD-MX procedure, produced using 1.63…
3QAL 1.7 Å, Crystal Structure of Arg280Ala mutant of Catalytic subunit of cAMP-dependent Protein…
3X2W 1.7 Å, Michaelis complex of cAMP-dependent Protein Kinase Catalytic Subunit

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