2V8N: Wild-type Structure of Lactose Permease

Wild-type Structure of Lactose Permease. Determined by X-ray diffraction at 3.6 Å resolution. Released 11 Sept 2007.

Method: X-ray diffraction
Resolution: 3.6 Å
Organism: ESCHERICHIA COLI
Chains: 2
Atoms: 6,584
Mol. weight: 93.06 kDa
Released: 11 Sept 2007

Explore 2V8N in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2V8N contains 66 α-helices and 0 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 33 helices, 0 β-strands

Element	Residues	Length
α-helix	7-38	32
α-helix	42-45	4
α-helix	48-57	10
α-helix	60-63	4
α-helix	64-68	5
α-helix	77-84	8
α-helix	91-95	5
α-helix	96-99	4
α-helix	104-109	6
α-helix	112-114	3
α-helix	122-136	15
α-helix	149-158	10
α-helix	161-164	4
α-helix	166-175	10
α-helix	178-183	6
α-helix	210-216	7
α-helix	220-227	8
α-helix	228-233	6
α-helix	234-240	7
α-helix	244-249	6
α-helix	254-263	10
α-helix	265-286	22
α-helix	288-308	21
α-helix	317-320	4
α-helix	326-337	12
α-helix	338-340	3
α-helix	343-345	3
α-helix	346-348	3
α-helix	349-353	5
α-helix	358-376	19
α-helix	378-388	11
α-helix	390-393	4
α-helix	396-399	4

Chain B: 33 helices, 0 β-strands

Element	Residues	Length
α-helix	7-38	32
α-helix	42-45	4
α-helix	48-57	10
α-helix	60-63	4
α-helix	64-68	5
α-helix	77-84	8
α-helix	91-95	5
α-helix	96-99	4
α-helix	104-109	6
α-helix	112-114	3
α-helix	122-136	15
α-helix	149-158	10
α-helix	161-164	4
α-helix	166-175	10
α-helix	178-183	6
α-helix	210-216	7
α-helix	220-228	9
α-helix	229-233	5
α-helix	234-240	7
α-helix	244-249	6
α-helix	254-263	10
α-helix	265-286	22
α-helix	288-308	21
α-helix	317-320	4
α-helix	326-337	12
α-helix	338-340	3
α-helix	343-345	3
α-helix	346-348	3
α-helix	349-353	5
α-helix	358-376	19
α-helix	378-388	11
α-helix	390-393	4
α-helix	396-399	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Lactose permease	A, B	protein	417	ESCHERICHIA COLI	P02920 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>2V8N_1 LACTOSE PERMEASE (chains A, B)
MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQ
PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA
GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL
AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD
QQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS
VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL
AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA

Primary citation

Structural Determination of Wild-Type Lactose Permease. Guan, L., Mirza, O., Verner, G. et al. Proc Natl Acad Sci U S A (2007) 104:15294. DOI 10.1073/PNAS.0707688104 · PubMed

Other PDB entries of the same protein (UniProt P02920 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9XOU 2.5 Å, CryoEM structure of LacY with Trimbody
6VBG 2.8 Å, Lactose permease complex with thiodigalactoside and nanobody 9043
2CFQ 2.95 Å, Sugar Free Lactose Permease at neutral pH
8YA0 2.97 Å, Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C)
6C9W 3.0 Å, Crystal Structure of a ligand bound LacY/Nanobody Complex
8YA3 3.27 Å, Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C) treated with DTT
8Y9Y 3.29 Å, Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+1C)
2CFP 3.3 Å, Sugar Free Lactose Permease at acidic pH
5GXB 3.3 Å, crystal structure of a LacY/Nanobody complex
4ZYR 3.31 Å, Crystal structure of E. coli Lactose permease G46W/G262W bound to p-nitrophenyl…
2Y5Y 3.38 Å, Crystal structure of LacY in complex with an affinity inactivator
1PV6 3.5 Å, Crystal structure of lactose permease

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