2XRP: Tubulin beta-2B chain

Human Doublecortin N-DC Repeat (1MJD) and Mammalian Tubulin (1JFF and 3HKE) Docked into the 8-Angstrom Cryo-EM Map of Doublecortin- Stabilised Microtubules. Determined by electron microscopy at 8.2 Å resolution. Released 24 Nov 2010.

Method: Electron microscopy
Resolution: 8.2 Å
Organisms: BOS TAURUS, HOMO SAPIENS
Chains: 9
Atoms: 27,756
Mol. weight: 415.47 kDa
Ligands: GDP, GTP
Released: 24 Nov 2010

Explore 2XRP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2XRP contains 167 α-helices and 129 β-strands across 9 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, C, E and G: 21 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	4	1	1
β-strand	6-9	4	1
α-helix	12-26	15
β-strand	30	1	2
β-strand	36	1	2
α-helix	49-52	4
β-strand	55	1	3
β-strand	61	1	3
β-strand	65-69	5	1
α-helix	72-78	7
α-helix	83-85	3
α-helix	89-91	3
β-strand	92-94	3	1
α-helix	105-109	5
α-helix	111-126	16
β-strand	134-139	6	1
α-helix	144-148	5
α-helix	149-160	12
β-strand	165-170	6	1
α-helix	184-194	11
β-strand	200-202	3	1
α-helix	206-214	9
α-helix	224-242	19
β-strand	248	1	4
α-helix	252-259	8
β-strand	267-268	2	1
β-strand	269-271	3	4
α-helix	289-295	7
α-helix	307-309	3
β-strand	312-320	9	4
α-helix	325-338	14
β-strand	351-356	6	4
β-strand	375-381	7	4
α-helix	382-384	3
α-helix	385-399	15
α-helix	403-405	3
α-helix	406-409	4
α-helix	415-436	22

Chains B, D, F and H: 20 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	5
α-helix	10-26	17
β-strand	65-69	5	5
α-helix	73-78	6
β-strand	92-94	3	5
α-helix	103-107	5
α-helix	109-128	20
β-strand	134-139	6	5
α-helix	144-149	6
α-helix	150-160	11
β-strand	165	1	5
β-strand	167-170	4	5
α-helix	184-194	11
β-strand	200-203	4	5
α-helix	206-215	10
α-helix	224-242	19
α-helix	252-259	8
β-strand	269-271	3	5
β-strand	277	1	6
α-helix	284-287	4
α-helix	288-294	7
α-helix	298-300	3
β-strand	312-321	10	5
α-helix	325-336	12
β-strand	343	1	5
β-strand	351-355	5	5
β-strand	368	1	6
β-strand	373-381	9	5
α-helix	382-384	3
α-helix	385-399	15
α-helix	403-405	3
α-helix	406-409	4
α-helix	415-429	15
α-helix	431-434	4

Chain I: 3 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	53-59	7	25
β-strand	68-72	5	25
α-helix	80-91	12
β-strand	103-106	4	25
β-strand	112-113	2	25
α-helix	116-118	3
β-strand	123-128	6	25
α-helix	132-133	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Tubulin beta-2B chain	A, C, E, G	protein	445	BOS TAURUS	Q6B856 (AlphaFold model)
Tubulin alpha-1D chain	B, D, F, H	protein	452	BOS TAURUS	Q2HJ86 (AlphaFold model)
Neuronal migration protein doublecortin	I	protein	95	HOMO SAPIENS	O43602 (AlphaFold model)

Sequence of entity 1 (A, C, E, G), FASTA

>2XRP_1 TUBULIN BETA-2B CHAIN (chains A, C, E, G)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM
AACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATADEQGEFEEEEGEDEA

Sequence of entity 2 (B, D, F, H), FASTA

>2XRP_2 TUBULIN ALPHA-1D CHAIN (chains B, D, F, H)
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFSVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRGHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYEPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEEGEEY

Sequence of entity 3 (I), FASTA

>2XRP_3 NEURONAL MIGRATION PROTEIN DOUBLECORTIN (chains I)
LSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIY
TIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTK

Ligands and cofactors

ID	Name	Formula	Copies
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	4
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	4

Primary citation

Template-Free 13-Protofilament Microtubule-Map Assembly Visualized at 8 A Resolution. Fourniol, F.J., Sindelar, C.V., Amigues, B. et al. J Cell Biol (2010) 191:463. DOI 10.1083/JCB.201007081 · PubMed

Other PDB entries of the same protein (UniProt Q6B856 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6S8K 1.52 Å, Structure, Thermodynamics, and Kinetics of Plinabulin Binding to two Tubulin Isotypes
8QL2 1.7 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
6ZWB 1.75 Å, Z-SBTub3 photoswitch bound to tubulin-DARPin D1 complex
4I4T 1.8 Å, Crystal structure of tubulin-RB3-TTL-Zampanolide complex
5IYZ 1.8 Å, Tubulin-MMAE complex
5NQU 1.8 Å, Tubulin Darpin cryo structure
7YZ3 1.8 Å, Molecular snapshots of drug release from tubulin: Apo state
8QEA 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
8QL3 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
8QL4 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
8QL5 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
8QL6 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…

Browse structure collections

About this viewer

MolViewer shows 2XRP directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.