2Y4I: KSR2-MEK1 heterodimer

KSR2-MEK1 heterodimer. Determined by X-ray diffraction at 3.46 Å resolution. Released 19 Jan 2011.

Method: X-ray diffraction
Resolution: 3.46 Å
Organisms: HOMO SAPIENS, ORYCTOLAGUS CUNICULUS
Chains: 2
Atoms: 4,661
Mol. weight: 81.55 kDa
Ligands: ATP, MG
Released: 19 Jan 2011

Explore 2Y4I in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2Y4I contains 34 α-helices and 29 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 15 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	656-658	3
β-strand	666-667	2	1
β-strand	671	1	2
β-strand	679-683	5	2
β-strand	684-685	2	1
β-strand	689-693	5	2
α-helix	696-697	2
α-helix	704-708	5
α-helix	711-714	4
β-strand	722	1	3
β-strand	727-730	4	2
β-strand	735-738	4	2
β-strand	740	1	2
α-helix	741-742	2
β-strand	745-746	2	3
α-helix	747-750	4
α-helix	761-779	19
β-strand	792-794	3	3
β-strand	800-801	2	3
β-strand	823	1	4
α-helix	827-830	4
α-helix	834-837	4
β-strand	840	1	5
α-helix	853-869	17
α-helix	879-887	9
α-helix	891-893	3
α-helix	903-911	9
α-helix	919-920	2
α-helix	921-928	8

Chain C: 19 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	44-55	12
β-strand	68-69	2	6
β-strand	73-75	3	7
β-strand	82-83	2	7
β-strand	86-87	2	6
β-strand	92	1	6
β-strand	95-100	6	7
α-helix	105-115	11
α-helix	116-120	5
β-strand	123	1	8
β-strand	126	1	8
β-strand	129-133	5	7
β-strand	138-143	6	7
β-strand	150	1	8
α-helix	151-156	6
α-helix	163-184	22
α-helix	193-195	3
β-strand	196-198	3	8
β-strand	204-206	3	8
α-helix	213-218	6
β-strand	223	1	4
α-helix	232-236	5
α-helix	242-258	17
α-helix	265-266	2
α-helix	271-273	3
β-strand	309	1	5
α-helix	310-319	10
α-helix	321-323	3
α-helix	325-326	2
α-helix	332-341	10
α-helix	350-351	2
α-helix	352-356	5
α-helix	359-366	8
α-helix	371-375	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Kinase suppressor of ras 2	B	protein	319	HOMO SAPIENS	Q6VAB6 (AlphaFold model)
Dual specificity mitogen-activated protein kinase kinase 1	C	protein	395	ORYCTOLAGUS CUNICULUS	P29678 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>2Y4I_1 KINASE SUPPRESSOR OF RAS 2 (chains B)
GPEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAI
RLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVV
RDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISG
VLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHARE
WPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKL
PKRNRRLSHPGHFWKSAEL

Sequence of entity 2 (C), FASTA

>2Y4I_2 DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 1 (chains C)
GPMPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLLELELDEQQRKRLEAFLTQKQ
KVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVL
HECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLT
YLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGT
HYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLS
SYGMDSRPPMAIFELLDYIVNEPPPKLPSAVFSLEFQDFVNKCLIKNPAERADLKQLMVH
AFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	2
MG	Magnesium ion	Mg	2

Water and common crystallization additives (CL) are not listed.

Primary citation

A Raf-Induced Allosteric Transition of Ksr Stimulates Ksr and Raf Phosphorylation of Mek. Brennan, D.F., Dar, A.C., Hertz, N.T. et al. Nature (2011) 472:366. DOI 10.1038/NATURE09860 · PubMed

Other PDB entries of the same protein (UniProt Q6VAB6 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7JUR 2.82 Å, Crystal Structure of KSR2:MEK1 in complex with AMP-PNP, and allosteric MEK inhibitor…
7JUS 2.99 Å, Crystal Structure of KSR2:MEK1 in complex with AMP-PNP, and allosteric MEK inhibitor…
7JUT 3.09 Å, Crystal Structure of KSR2:MEK1 in complex with ANP-PNP, and allosteric MEK inhibitor…
7JUU 3.19 Å, Crystal Structure of KSR2:MEK1 in complex with AMP-PNP, and allosteric MEK inhibitor…
7JUQ 3.22 Å, Crystal Structure of KSR2:MEK1 in complex with ADP
7UMB 3.23 Å, NanoBRET tracer Tram-bo bound to a KSR2-MEK1 complex
7JUV 3.36 Å, Crystal Structure of KSR2:MEK1 in complex with AMP-PNP, and allosteric MEK inhibitor…
5KKR 3.51 Å, KSR2:MEK1 Complex Bound to the Small Molecule APS-2-79

Browse structure collections

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