33CG: MAGE-A4 (230-239)-bound HLA-A*02:01
Cryo-EM structure of MAGE-A4 (230-239)-bound HLA-A*02:01 in complex with Fab VR-6. Determined by electron microscopy at 2.2 Å resolution. Released 26 Aug 2026.
- Method
- Electron microscopy
- Resolution
- 2.2 Å
- Organisms
- Homo sapiens, Mus musculus
- Chains
- 7
- Atoms
- 6,667
- Mol. weight
- 147.37 kDa
- Released
- 26 Aug 2026
Explore 33CG in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
33CG contains 29 α-helices and 75 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 11 helices, 19 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-12 | 10 | 1 |
| α-helix | 20 | 1 | |
| β-strand | 21-28 | 8 | 1 |
| β-strand | 31-37 | 7 | 1 |
| β-strand | 46-47 | 2 | 1 |
| α-helix | 52-54 | 3 | |
| α-helix | 57-84 | 28 | |
| β-strand | 94-103 | 10 | 1 |
| β-strand | 109-118 | 10 | 1 |
| β-strand | 121-126 | 6 | 1 |
| β-strand | 133-135 | 3 | 1 |
| α-helix | 138-149 | 12 | |
| α-helix | 152-158 | 7 | |
| α-helix | 159-163 | 5 | |
| α-helix | 164-174 | 11 | |
| α-helix | 176-179 | 4 | |
| β-strand | 183 | 1 | 2 |
| α-helix | 184-185 | 2 | |
| β-strand | 186-193 | 8 | 3 |
| β-strand | 198-208 | 11 | 3 |
| β-strand | 209 | 1 | 2 |
| β-strand | 213-219 | 7 | 4 |
| β-strand | 222-224 | 3 | 4 |
| β-strand | 228-230 | 3 | 3 |
| α-helix | 231-233 | 3 | |
| β-strand | 234-235 | 2 | 3 |
| β-strand | 241-250 | 10 | 3 |
| α-helix | 254-256 | 3 | |
| β-strand | 257-263 | 7 | 4 |
| β-strand | 270-272 | 3 | 4 |
Chain B: 3 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3 | 1 | 5 |
| α-helix | 4-5 | 2 | |
| β-strand | 6-11 | 6 | 6 |
| β-strand | 21-30 | 10 | 6 |
| β-strand | 31 | 1 | 5 |
| β-strand | 36-41 | 6 | 7 |
| β-strand | 44-45 | 2 | 7 |
| α-helix | 46 | 1 | |
| β-strand | 50-51 | 2 | 6 |
| α-helix | 52-54 | 3 | |
| β-strand | 55-56 | 2 | 6 |
| β-strand | 62-70 | 9 | 6 |
| β-strand | 78-83 | 6 | 7 |
| β-strand | 91-94 | 4 | 7 |
Chain D: 4 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4-7 | 4 | 8 |
| β-strand | 10-13 | 4 | 9 |
| β-strand | 19-29 | 11 | 8 |
| α-helix | 31-33 | 3 | |
| β-strand | 34-39 | 6 | 9 |
| α-helix | 44-45 | 2 | |
| β-strand | 46-50 | 5 | 9 |
| β-strand | 54-55 | 2 | 9 |
| α-helix | 56 | 1 | |
| β-strand | 63-76 | 14 | 8 |
| α-helix | 81-83 | 3 | |
| β-strand | 86-91 | 6 | 9 |
| β-strand | 99-100 | 2 | 9 |
| β-strand | 104-108 | 5 | 9 |
Chain E: 3 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 10 |
| β-strand | 11-12 | 2 | 11 |
| β-strand | 18-25 | 8 | 10 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 12 |
| β-strand | 45-51 | 7 | 12 |
| β-strand | 58-60 | 3 | 12 |
| β-strand | 69-73 | 5 | 10 |
| β-strand | 78-83 | 6 | 10 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-99 | 8 | 12 |
| α-helix | 100 | 1 | |
| β-strand | 106-109 | 4 | 12 |
| β-strand | 113-115 | 3 | 12 |
| β-strand | 116-117 | 2 | 11 |
Chain F: 4 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4-7 | 4 | 13 |
| β-strand | 10-13 | 4 | 14 |
| β-strand | 19-25 | 7 | 13 |
| β-strand | 33-38 | 6 | 14 |
| β-strand | 45-49 | 5 | 14 |
| β-strand | 53-54 | 2 | 14 |
| α-helix | 55 | 1 | |
| β-strand | 62-67 | 6 | 13 |
| β-strand | 70-75 | 6 | 13 |
| α-helix | 80-82 | 3 | |
| β-strand | 85-90 | 6 | 14 |
| α-helix | 97 | 1 | |
| β-strand | 98 | 1 | 14 |
| α-helix | 99 | 1 | |
| β-strand | 102-106 | 5 | 14 |
Chain G: 4 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-6 | 4 | 15 |
| α-helix | 7-9 | 3 | |
| β-strand | 10-12 | 3 | 16 |
| β-strand | 18-25 | 8 | 15 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 16 |
| β-strand | 45-52 | 8 | 16 |
| β-strand | 57-60 | 4 | 16 |
| α-helix | 62-64 | 3 | |
| β-strand | 65 | 1 | 15 |
| β-strand | 68-73 | 6 | 15 |
| β-strand | 78-83 | 6 | 15 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-100 | 9 | 16 |
| β-strand | 105-109 | 5 | 16 |
| β-strand | 113-117 | 5 | 16 |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| MHC class I antigen | A | protein | 296 | Homo sapiens | F6IQS1 (AlphaFold model) |
| Beta-2-microglobulin | B | protein | 100 | Homo sapiens | P61769 (AlphaFold model) |
| Melanoma-associated antigen 4 | C | protein | 10 | Homo sapiens | P43358 (AlphaFold model) |
| Fab VR-6 light chain | D | protein | 216 | Homo sapiens | |
| Fab VR-6 heavy chain | E | protein | 274 | Homo sapiens | |
| Fab BBM.1 light chain | F | protein | 214 | Mus musculus | |
| Fab BBM.1 heavy chain | G | protein | 222 | Mus musculus | |
Sequence of entity 1 (A), FASTA
>33CG_1 MHC class I antigen (chains A)
MGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEY
WDGETRKVKAHSQTHRVDLGTLRGCYNQSEAGSHTVQRMYGCDVGSDWRFLRGYHQYAYD
GKDYIALKEDLRSWTAADMCAQTTKHKWEAAHVAEQLRAYLEGTCVEWLRRYLENGKETL
QRTDAPKTHMTHHAVSDHEATLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDG
TFQKWAAVVVPSGQEQRYTCHVQHEGLPKPLTLRWEPGSGSGLNDIFEAQKIEWHE
Sequence of entity 2 (B), FASTA
>33CG_2 Beta-2-microglobulin (chains B)
MIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKD
WSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Sequence of entity 3 (C), FASTA
>33CG_3 Melanoma-associated antigen 4 (chains C)
GVYDGREHTV
Sequence of entity 4 (D), FASTA
>33CG_4 Fab VR-6 light chain (chains D)
EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIP
DRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGTSPALTFGGGTKVEIKRTVAAPSVFIF
PPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSST
LTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
Sequence of entity 5 (E), FASTA
>33CG_5 Fab VR-6 heavy chain (chains E)
QVQLQESGGGLVKPGGSLRLSCAASGFTFSSYSMNWVRQAPGRGLEWVSSISSRSSFMYY
ADSVKGRFTISRDNAKNSLYLQMSSLRAEDTAVFYCARETWNYGAFDIWGQGTMVTVSSA
STKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSG
LYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCAAAEQKLISEEDLNEMEQ
KLISEEDLNEMEQKLISEEDLNEMAAHHHHHHHH
Sequence of entity 6 (F), FASTA
>33CG_6 Fab BBM.1 light chain (chains F)
DIQMTQSPASQSASLGESVTITCLASQTIGTWLAWYQQKPGKSPQLLIYAATSLADGVPS
RFSGSGSGTKFSLKIRTLQAEDFVSYYCQQLYSKPYTFGGGTKLEIKRTVAAPSVFIFPP
SDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLT
LSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
Sequence of entity 7 (G), FASTA
>33CG_7 Fab BBM.1 heavy chain (chains G)
EVQLQQSGAELVKPGASVKLSCTPSGFNVKDTYIHWVKQRPKQGLEWIGRIDPSDGDIKY
DPKFQGKATITADTSSNTVSLQLSSLTSEDTAVYYCARWFGDYGAMNYWGQGTSVTVSSA
STKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSG
LYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC
Primary citation
Systematic De-Risking of TCR-Mimic Therapeutics Through Proteome-Wide Off-Target Landscaping and a Generalizable Design Rule Framework. Schuster, S., Hartl, F.A., Stehle, J. et al. To be published.
Other PDB entries of the same protein (UniProt F6IQS1 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 6TDP 1.4 Å, Crystal structure of the disulfide engineered HLA-A0201 molecule in complex with one GL…
- 6TDQ 1.6 Å, Crystal structure of the disulfide engineered HLA-A0201 molecule in complex with one GM…
- 6TDO 1.65 Å, Crystal structure of the disulfide engineered HLA-A0201 molecule in complex with one GM…
- 6TDS 1.7 Å, Crystal structure of the disulfide engineered HLA-A0201 molecule without peptide bound…
- 6TDR 1.75 Å, Crystal structure of the disulfide engineered HLA-A0201 molecule devoid of peptide…
- 33CI 2.2 Å, Cryo-EM structure of MAGE-A4 (230-239)-bound HLA-A*02:01 in complex with Fab VR-58
- 33CH 2.4 Å, Cryo-EM structure of MAGE-A4 (230-239)-bound HLA-A*02:01 in complex with Fab VR-4
Browse structure collections
About this viewer
MolViewer shows 33CG directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.