3GWU: Leucine transporter LeuT

Leucine transporter LeuT in complex with sertraline. Determined by X-ray diffraction at 2.14 Å resolution. Released 12 May 2009.

Method: X-ray diffraction
Resolution: 2.14 Å
Organism: Aquifex aeolicus
Chains: 1
Atoms: 4,175
Mol. weight: 58.09 kDa
Ligands: LEU, SRE
Released: 12 May 2009

Explore 3GWU in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3GWU contains 38 α-helices and 4 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 38 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	11-22	12
α-helix	25	1
α-helix	26-30	5
α-helix	31-37	7
α-helix	41-50	10
α-helix	51-55	5
α-helix	56-71	16
α-helix	77-84	8
α-helix	88-94	7
α-helix	96-123	28
α-helix	137-152	16
β-strand	161	1	1
α-helix	166-183	18
α-helix	187-192	6
α-helix	193-214	22
β-strand	217-218	2	2
β-strand	221-222	2	2
α-helix	223-231	9
α-helix	237-239	3
α-helix	241-255	15
α-helix	261-266	6
α-helix	276-287	12
α-helix	288-294	7
α-helix	298-306	9
α-helix	308-316	9
α-helix	321	1
α-helix	322-326	5
α-helix	327-331	5
α-helix	337-369	33
α-helix	375-395	21
β-strand	396	1	1
α-helix	399-405	7
α-helix	406-411	6
α-helix	412-421	10
α-helix	422-426	5
α-helix	429-437	9
α-helix	443-445	3
α-helix	447-450	4
α-helix	451-455	5
α-helix	456-470	15
α-helix	473-476	4
α-helix	483-514	32

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Transporter	A	protein	515	Aquifex aeolicus	O67854 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>3GWU_1 Transporter (chains A)
MEVKREHWATRLGLILAMAGNAVGLGNFLRFPVQAAENGGGAFMIPYIIAFLLVGIPLMW
IEWAMGRYGGAQGHGTTPAIFYLLWRNRFAKILGVFGLWIPLVVAIYYVYIESWTLGFAI
KFLVGLVPEPPPNATDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFINV
SILIRGISKGIERFAKIAMPTLFILAVFLVIRVFLLETPNGTAADGLNFLWTPDFEKLKD
PGVWIAAVGQIFFTLSLGFGAIITYASYVRKDQDIVLSGLTAATLNEKAEVILGGSISIP
AAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAGGTFLGFLWFFLLFFAGLTSSIAIM
QPMIAFLEDELKLSRKHAVLWTAAIVFFSAHLVMFLNKSLDEMDFWAGTIGVVFFGLTEL
IIFFWIFGADKAWEEINRGGIIKVPRIYYYVMRYITPAFLAVLLVVWAREYIPKIMEETH
WTVWITRFYIIGLFLFLTFLVFLAERRRNHESAGT

Ligands and cofactors

ID	Name	Formula	Copies
LEU	Leucine	C6 H13 N O2	1
SRE	(1S,4S)-4-(3,4-dichlorophenyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine	C17 H17 Cl2 N	1

Water and common crystallization additives (NA) are not listed.

Primary citation

Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures. Zhou, Z., Zhen, J., Karpowich, N.K. et al. Nat Struct Mol Biol (2009) 16:652-657. DOI 10.1038/nsmb.1602 · PubMed

Other PDB entries of the same protein (UniProt O67854 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2A65 1.65 Å, Crystal structure of LEUTAA, a bacterial homolog of Na+/Cl--dependent neurotransmitter…
2Q72 1.7 Å, Crystal Structure Analysis of LeuT complexed with L-leucine, sodium, and imipramine
3F3E 1.8 Å, Crystal structure of LeuT bound to L-leucine (30 mM) and sodium
2Q6H 1.85 Å, Crystal Structure Analysis of LeuT complexed with L-leucine, sodium, and clomipramine
2QEI 1.85 Å, Crystal structure analysis of LeuT complexed with L-alanine, sodium, and clomipramine
2QB4 1.9 Å, Crystal Structure Analysis of LeuT complexed with L-leucine, sodium and desipramine
3F48 1.9 Å, Crystal structure of LeuT bound to L-alanine and sodium
3F4I 1.95 Å, Crystal Structure of LeuT bound to L-selenomethionine and sodium
3F3A 2.0 Å, Crystal Structure of LeuT bound to L-Tryptophan and Sodium
3GJD 2.0 Å, Crystal Structure of LeuT with bound OG
3F3C 2.1 Å, Crystal structure of LeuT bound to 4-Fluoro-L-Phenylalanine and sodium
3USP 2.1 Å, Crystal structure of LeuT in heptyl-beta-D-Selenoglucoside

Browse structure collections

About this viewer

MolViewer shows 3GWU directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.