3LUK: MID domain from hAGO2

Crystal structure of MID domain from hAGO2. Determined by X-ray diffraction at 1.7 Å resolution. Released 26 May 2010.

Method: X-ray diffraction
Resolution: 1.7 Å
Organism: Homo sapiens
Chains: 3
Atoms: 3,738
Mol. weight: 47.39 kDa
Ligands: PO4
Released: 26 May 2010

Explore 3LUK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3LUK contains 29 α-helices and 18 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	441-444	4
β-strand	448	1	1
β-strand	451-455	5	2
α-helix	464-481	18
α-helix	484	1
β-strand	485	1	1
α-helix	486	1
β-strand	491-494	4	2
α-helix	498-500	3
α-helix	501-511	11
β-strand	517-522	6	2
α-helix	528-533	6
α-helix	534-540	7
β-strand	544-548	5	2
α-helix	549-553	5
α-helix	557-572	16

Chain B: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	448	1	3
β-strand	451-455	5	4
α-helix	464-480	17
α-helix	484	1
β-strand	485	1	3
α-helix	486	1
β-strand	491-494	4	4
α-helix	498-500	3
α-helix	501-511	11
β-strand	517-522	6	4
α-helix	527-533	7
α-helix	534-539	6
β-strand	544-548	5	4
α-helix	550-553	4
α-helix	557-571	15

Chain C: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	442-444	3
β-strand	448	1	5
β-strand	451-455	5	6
α-helix	464-480	17
α-helix	484	1
β-strand	485	1	5
α-helix	486	1
β-strand	491-494	4	6
α-helix	498-500	3
α-helix	501-511	11
β-strand	517-522	6	6
α-helix	528-533	6
α-helix	534-540	7
β-strand	544-548	5	6
α-helix	549-553	5
α-helix	557-572	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein argonaute-2	A, B, C	protein	138	Homo sapiens	Q9UKV8 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>3LUK_1 Protein argonaute-2 (chains A, B, C)
SNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQG
ADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTP
QTLSNLCLKINVKLGGVN

Ligands and cofactors

ID	Name	Formula	Copies
PO4	Phosphate ion	O4 P	8

Water and common crystallization additives (GOL) are not listed.

Primary citation

Structural basis for 5'-nucleotide base-specific recognition of guide RNA by human AGO2. Frank, F., Sonenberg, N., Nagar, B. Nature (2010) 465:818-822. DOI 10.1038/nature09039 · PubMed

Other PDB entries of the same protein (UniProt Q9UKV8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9BF2 1.59 Å, MID domain of Ago2 bound to UMP
4Z4D 1.6 Å, Human Argonaute2 Bound to t1-G Target RNA
3LUC 1.69 Å, Crystal structure of MID domain from hAGO2
7C6B 1.7 Å, Crystal structure of Ago2 MID domain in complex with 6-(3-(2-carboxyethyl)phenyl)purine…
9LSN 1.75 Å, hAGO2-MID in complex with a chemical modified uridine monophosphate
9BF0 1.78 Å, MID domain of human Argo2 bound to UTP
3LUJ 1.8 Å, Crystal structure of MID domain from hAGO2 in complex with UMP
4Z4E 1.8 Å, Human Argonaute2 Bound to t1-U Target RNA
9LMZ 1.8 Å, hAGO2-MID in complex with a chemical modified uridine monophosphate
4W5O 1.8 Å, The Crystal Structure of Human Argonaute2 Bound to a Guide and Target RNA Containing…
3LUG 1.85 Å, Crystal structure of MID domain from hAGO2 in complex with CMP
6RA4 1.9 Å, Human argonaute-2 paz domain (214-347) in complex with cgugacucu

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