3MQ9: PDB entry 3MQ9

Crystal Structure of Ectodomain Mutant of BST-2/Tetherin/CD317 Fused to MBP. Determined by X-ray diffraction at 2.8 Å resolution. Released 27 Oct 2010.

Method: X-ray diffraction
Resolution: 2.8 Å
Organisms: Escherichia coli, Homo sapiens
Chains: 8
Atoms: 28,225
Mol. weight: 417.55 kDa
Released: 27 Oct 2010

Explore 3MQ9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3MQ9 contains 197 α-helices and 184 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 24 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	7-10	4	1
α-helix	17-31	15
β-strand	35-38	4	1
α-helix	43-51	9
β-strand	59-63	5	1
α-helix	66-72	7
β-strand	76	1	2
α-helix	77-79	3
α-helix	83-86	4
β-strand	89	1	3
α-helix	91-94	4
α-helix	95-97	3
β-strand	98-99	2	4
β-strand	102-103	2	4
β-strand	105-112	8	1
β-strand	114-118	5	5
β-strand	128	1	6
α-helix	129-131	3
α-helix	132-140	9
β-strand	145-147	3	5
α-helix	154-161	8
α-helix	162-164	3
β-strand	167-171	5	7
β-strand	176-182	7	7
α-helix	186-200	15
α-helix	210-218	9
β-strand	222-227	6	5
α-helix	229-231	3
α-helix	232-236	5
β-strand	242-245	4	5
α-helix	246-248	3
β-strand	249	1	6
β-strand	250	1	8
β-strand	253	1	8
α-helix	254-255	2
β-strand	258-266	9	1
β-strand	267	1	2
α-helix	273-278	6
α-helix	279-284	6
α-helix	287-296	10
β-strand	302	1	1
β-strand	304	1	3
α-helix	305-311	7
α-helix	315-325	11
β-strand	328-329	2	1
α-helix	336-352	17
α-helix	357-456	100

Chain B: 24 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	7-10	4	9
α-helix	17-31	15
β-strand	35-38	4	9
α-helix	43-51	9
β-strand	59-63	5	9
α-helix	65-72	8
β-strand	76	1	10
α-helix	77-79	3
α-helix	83-86	4
β-strand	89	1	11
α-helix	91-94	4
α-helix	95-97	3
β-strand	98-99	2	12
β-strand	102-103	2	12
β-strand	105-112	8	9
β-strand	114-118	5	13
β-strand	128	1	14
α-helix	129-131	3
α-helix	132-140	9
β-strand	145-147	3	13
α-helix	154-163	10
β-strand	167-171	5	15
β-strand	176-182	7	15
α-helix	186-200	15
α-helix	210-218	9
β-strand	222-227	6	13
α-helix	229-231	3
α-helix	232-236	5
β-strand	242-245	4	13
α-helix	246-248	3
β-strand	249	1	14
β-strand	250	1	16
β-strand	253	1	16
α-helix	254-255	2
β-strand	258-266	9	9
β-strand	267	1	10
α-helix	273-278	6
α-helix	279-284	6
α-helix	287-296	10
β-strand	302	1	9
β-strand	304	1	11
α-helix	305-311	7
α-helix	315-326	12
β-strand	328-329	2	9
α-helix	336-352	17
α-helix	357-367	11
α-helix	370-456	87

Chain C: 25 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	7-11	5	17
α-helix	17-30	14
β-strand	35-38	4	17
α-helix	43-50	8
α-helix	51-53	3
β-strand	59-63	5	17
α-helix	66-72	7
β-strand	76	1	18
α-helix	77-79	3
α-helix	83-86	4
β-strand	89	1	19
α-helix	91-95	5
β-strand	98-99	2	20
β-strand	102-103	2	20
β-strand	106-111	6	17
β-strand	114-118	5	21
β-strand	128	1	22
α-helix	129-131	3
α-helix	132-140	9
β-strand	145-147	3	21
α-helix	154-162	9
β-strand	167-171	5	23
β-strand	176-182	7	23
α-helix	186-200	15
α-helix	210-218	9
β-strand	222-227	6	21
α-helix	229-231	3
α-helix	232-238	7
β-strand	242-245	4	21
α-helix	246-248	3
β-strand	249	1	22
β-strand	250	1	24
β-strand	253	1	24
α-helix	257	1
β-strand	258-259	2	25
β-strand	260-266	7	17
β-strand	267	1	18
α-helix	273-279	7
α-helix	280-284	5
α-helix	287-296	10
β-strand	301-302	2	17
β-strand	304	1	19
α-helix	305-311	7
α-helix	315-325	11
β-strand	328-329	2	25
α-helix	330-331	2
α-helix	336-352	17
α-helix	357-366	10
α-helix	372-453	82

Chain D: 26 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	7-11	5	26
α-helix	17-30	14
β-strand	35-38	4	26
α-helix	43-51	9
β-strand	59-63	5	26
α-helix	66-72	7
β-strand	76	1	27
α-helix	77-79	3
α-helix	83-86	4
β-strand	89	1	28
α-helix	91-93	3
α-helix	95-97	3
β-strand	98-99	2	29
β-strand	102-103	2	29
β-strand	105-112	8	26
β-strand	114-118	5	30
β-strand	128	1	31
α-helix	129-131	3
α-helix	132-140	9
β-strand	145-147	3	30
α-helix	154-161	8
α-helix	162-164	3
β-strand	167-171	5	32
β-strand	176-182	7	32
α-helix	186-200	15
α-helix	210-218	9
β-strand	222-227	6	30
α-helix	229-231	3
α-helix	233-238	6
β-strand	242-245	4	30
α-helix	246-248	3
β-strand	249-250	2	31
β-strand	253-254	2	31
α-helix	255	1
β-strand	258-266	9	26
β-strand	267	1	27
α-helix	273-279	7
α-helix	280-284	5
α-helix	287-296	10
β-strand	301-302	2	26
β-strand	304	1	28
α-helix	305-311	7
α-helix	315-325	11
β-strand	328-329	2	26
α-helix	330-331	2
α-helix	336-352	17
α-helix	357-368	12
α-helix	375-453	79

Chain E: 23 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	7-10	4	33
α-helix	17-31	15
β-strand	35-38	4	33
α-helix	43-51	9
β-strand	59-63	5	33
α-helix	66-72	7
β-strand	76	1	34
α-helix	77-79	3
α-helix	83-86	4
β-strand	89	1	35
α-helix	91-94	4
α-helix	95-97	3
β-strand	98-99	2	36
β-strand	102-103	2	36
β-strand	105-112	8	33
β-strand	114-118	5	37
β-strand	128	1	38
α-helix	129-131	3
α-helix	132-140	9
β-strand	145-147	3	37
α-helix	154-161	8
β-strand	167-171	5	39
β-strand	176-182	7	39
α-helix	186-200	15
α-helix	210-218	9
β-strand	222-227	6	37
α-helix	229-231	3
α-helix	232-236	5
β-strand	242-245	4	37
α-helix	246-248	3
β-strand	249	1	38
β-strand	250	1	40
β-strand	253	1	40
α-helix	254-255	2
β-strand	258-266	9	33
β-strand	267	1	34
α-helix	273-278	6
α-helix	279-284	6
α-helix	287-296	10
β-strand	302	1	33
β-strand	304	1	35
α-helix	305-311	7
α-helix	315-325	11
β-strand	328-329	2	33
α-helix	336-352	17
α-helix	357-456	100

Chain F: 24 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	7-10	4	41
α-helix	17-31	15
β-strand	35-38	4	41
α-helix	43-51	9
β-strand	59-63	5	41
α-helix	66-72	7
β-strand	76	1	42
α-helix	77-79	3
α-helix	83-86	4
β-strand	89	1	43
α-helix	91-94	4
α-helix	95-97	3
β-strand	98-99	2	44
β-strand	102-103	2	44
β-strand	105-112	8	41
β-strand	114-118	5	45
β-strand	128	1	46
α-helix	129-131	3
α-helix	132-140	9
β-strand	145-147	3	45
α-helix	154-161	8
β-strand	167-171	5	47
β-strand	176-182	7	47
α-helix	186-200	15
α-helix	210-218	9
β-strand	222-227	6	45
α-helix	229-231	3
α-helix	232-236	5
β-strand	242-245	4	45
α-helix	246-248	3
β-strand	249	1	46
β-strand	250	1	48
β-strand	253	1	48
α-helix	254-255	2
β-strand	258-266	9	41
β-strand	267	1	42
α-helix	273-278	6
α-helix	279-284	6
α-helix	287-296	10
β-strand	302	1	41
β-strand	304	1	43
α-helix	305-311	7
α-helix	315-325	11
β-strand	328-329	2	41
α-helix	336-352	17
α-helix	357-369	13
α-helix	372-456	85

Chain G: 26 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	6-11	6	49
α-helix	17-30	14
β-strand	34-38	5	49
α-helix	43-50	8
α-helix	51-53	3
β-strand	59-63	5	49
α-helix	66-71	6
β-strand	76	1	50
α-helix	77-79	3
α-helix	83-86	4
β-strand	89	1	51
α-helix	91-93	3
α-helix	95-97	3
β-strand	98-99	2	52
β-strand	102-103	2	52
β-strand	105-112	8	49
β-strand	114-118	5	53
β-strand	128	1	54
α-helix	129-131	3
α-helix	132-140	9
β-strand	145-147	3	53
α-helix	154-162	9
β-strand	167-171	5	55
β-strand	176-182	7	55
α-helix	186-200	15
α-helix	210-218	9
β-strand	222-227	6	53
α-helix	229-231	3
α-helix	232-238	7
β-strand	242-245	4	53
α-helix	246-248	3
β-strand	249	1	54
β-strand	250	1	56
β-strand	253	1	56
α-helix	254-255	2
β-strand	258-266	9	49
β-strand	267	1	50
α-helix	273-279	7
α-helix	280-284	5
α-helix	287-296	10
β-strand	301-302	2	49
β-strand	304	1	51
α-helix	305-311	7
α-helix	315-325	11
β-strand	328-329	2	49
α-helix	330-331	2
α-helix	336-351	16
α-helix	357-367	11
α-helix	375-453	79

Chain H: 25 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	6-11	6	57
α-helix	17-30	14
β-strand	34-38	5	57
α-helix	43-51	9
β-strand	59-63	5	57
α-helix	66-71	6
β-strand	76	1	58
α-helix	77-79	3
α-helix	83-86	4
β-strand	89	1	59
α-helix	91-97	7
β-strand	98-99	2	60
β-strand	102-103	2	60
β-strand	105-111	7	57
β-strand	114-118	5	61
β-strand	128	1	62
α-helix	129-131	3
α-helix	132-140	9
β-strand	145-147	3	61
α-helix	154-163	10
β-strand	169-171	3	63
β-strand	176-181	6	63
α-helix	186-200	15
α-helix	210-218	9
β-strand	222-227	6	61
α-helix	229-231	3
α-helix	232-238	7
β-strand	242-245	4	61
α-helix	246-248	3
β-strand	249-250	2	62
β-strand	253-254	2	62
α-helix	255	1
α-helix	257	1
β-strand	258-259	2	64
β-strand	260-266	7	57
β-strand	267	1	58
α-helix	273-279	7
α-helix	280-284	5
α-helix	287-296	10
β-strand	301-302	2	57
β-strand	304	1	59
α-helix	305-311	7
α-helix	315-325	11
β-strand	328-329	2	64
α-helix	330-331	2
α-helix	336-352	17
α-helix	357-368	12
α-helix	375-453	79

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Bone marrow stromal antigen 2 fused to Maltose-binding periplasmic protein	A, B, C, D, E, F, G, H	protein	471	Escherichia coli, Homo sapiens	P0AEX9 (AlphaFold model), Q10589 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F, G, H), FASTA

>3MQ9_1 Bone marrow stromal antigen 2 fused to Maltose-binding periplasmic protein (chains A, B, C, D, E, F, G, H)
MNTIHHHHHHNTSMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEK
FPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAY
PIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGG
YAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTI
NGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLT
DEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTA
VINAASGRQTVDEALKDAQTRITAARDGLRAVMEARNVTHLLQQELTEAQKGFQDVEAQA
ATANHTVMALMASLDAEKAQGQKKVEELEGEITTLNHKLQDASAEVERLRR

Primary citation

Structural insight into the mechanisms of enveloped virus tethering by tetherin. Yang, H., Wang, J., Jia, X. et al. Proc Natl Acad Sci U S A (2010) 107:18428-18432. DOI 10.1073/pnas.1011485107 · PubMed

Other PDB entries of the same protein (UniProt P0AEX9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8C8F 1.15 Å, Crystal structure of the E. coli maltodextrin-binding protein
4EXK 1.28 Å, A chimera protein containing MBP fused to the C-terminal domain of the uncharacterized…
3Q27 1.3 Å, Cyrstal structure of human alpha-synuclein (32-57) fused to maltose binding protein (MBP)
7KD4 1.31 Å, Structure of the C-terminal domain of the Menangle virus phosphoprotein (residues 329…
5M13 1.37 Å, Synthetic nanobody in complex with MBP
5HZ7 1.43 Å, High-resolution crystal structure of the minor DNA-binding pilin ComP from Neisseria…
5H7Q 1.45 Å, Crystal structure of human MNDA PYD domain with MBP tag
6XDS 1.47 Å, Crystal structure of MBP-TREM2 Ig domain fusion with fragment,…
4IRL 1.47 Å, X-ray structure of the CARD domain of zebrafish GBP-NLRP1 like protein
8SVY 1.47 Å, MBP-Mcl1 in complex with ligand 10
3MP6 1.48 Å, Complex Structure of Sgf29 and dimethylated H3K4
9CLC 1.48 Å, Crystal structure of maltose binding protein (Apo), mutant Trp10 to 4-Cyanotryptophan

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