3N7M: W1252A mutant of HCR D/C VPI 5995

Name: PDB 3N7M: Crystal structure of W1252A mutant of HCR D/C VPI 5995
Published: 2010-09-08
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: toxin

Crystal structure of W1252A mutant of HCR D/C VPI 5995. Determined by X-ray diffraction at 2.6 Å resolution. Released 8 Sept 2010.

Method: X-ray diffraction
Resolution: 2.6 Å
Organism: Clostridium botulinum
Chains: 1
Atoms: 3,470
Mol. weight: 49.11 kDa
Released: 8 Sept 2010

Explore 3N7M in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3N7M contains 12 α-helices and 36 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 36 β-strands

Element	Residues	Length	Sheet
α-helix	864-867	4
β-strand	868-875	8	1
β-strand	878-881	4	1
β-strand	888-892	5	2
β-strand	894	1	3
β-strand	896-897	2	1
α-helix	898	1
β-strand	905-908	4	1
β-strand	914	1	3
β-strand	916-920	5	2
α-helix	923-930	8
β-strand	933-943	11	1
α-helix	949-951	3
β-strand	952-958	7	2
β-strand	963-969	7	2
β-strand	972-978	7	2
β-strand	984-990	7	2
β-strand	1003-1011	9	1
β-strand	1015-1020	6	1
β-strand	1023-1029	7	1
β-strand	1041-1048	8	2
α-helix	1049	1
β-strand	1063-1072	10	1
α-helix	1078-1087	10
β-strand	1093	1	4
β-strand	1095	1	5
β-strand	1101	1	5
α-helix	1102	1
β-strand	1103	1	6
β-strand	1107-1112	6	7
α-helix	1113-1115	3
β-strand	1118-1123	6	7
β-strand	1126-1131	6	7
α-helix	1132	1
β-strand	1144-1150	7	7
β-strand	1157	1	6
β-strand	1159	1	4
β-strand	1163-1169	7	7
β-strand	1174-1179	6	7
β-strand	1190-1196	7	7
α-helix	1200-1206	7
β-strand	1209-1216	8	7
β-strand	1219-1227	9	7
β-strand	1234-1245	12	7
β-strand	1255-1263	9	7
α-helix	1270-1272	3
α-helix	1274-1276	3
β-strand	1278-1282	5	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Neurotoxin	A	protein	424	Clostridium botulinum	Q9LBR1

Sequence of entity 1 (A), FASTA

>3N7M_1 Neurotoxin (chains A)
NSINDSKILSLQNKKNTLMDTSGYNAEVRVEGNVQLNPIFPFDFKLGSSGDDRGKVIVTQ
NENIVYNAMYESFSISFWIRINKWVSNLPGYTIIDSVKNNSGWSIGIISNFLVFTLKQNE
NSEQDINFSYDISKNAAGYNKWFFVTITTNMMGNMMIYINGKLIDTIKVKELTGINFSKT
ITFQMNKIPNTGLITSDSDNINMWIRDFYIFAKELDDKDINILFNSLQYTNVVKDYWGND
LRYDKEYYMINVNYMNRYMSKKGNGIVFNTRKNNNDFNEGYKIIIKRIRGNTNDTRVRGE
NVLYFNTTIDNKQYSLGMYKPSRNLGTDLVPLGALDQPMDEIRKYGSFIIQPCNTFDYYA
SQLFLSSNATTNRLGILSIGSYSFKLGDDYAFNHEYLIPVIKIEHYASLLESTSTHWVFV
PASE

Primary citation

Identification of a Unique Ganglioside Binding Loop within Botulinum Neurotoxins C and D-SA . Karalewitz, A.P., Kroken, A.R., Fu, Z. et al. Biochemistry (2010) 49:8117-8126. DOI 10.1021/bi100865f · PubMed

Other PDB entries of the same protein (UniProt Q9LBR1), best resolution first:

3N7L 2.0 Å, Crystal structure of botulinum neurotoxin serotype D/C VPI 5993 binding domain
4ISR 2.59 Å, Binding domain of Botulinum neurotoxin DC in complex with rat synaptotagmin II
4ISQ 2.65 Å, Binding domain of Botulinum neurotoxin DC in complex with human synaptotagmin I
4FVV 2.7 Å, Crystal structure of HCR/D-Sa-GBL1/C
3AZW 2.99 Å, Crystal structure of the receptor binding domain
3AZV 3.1 Å, Crystal structure of the receptor binding domain

Browse structure collections

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