3QX9: MID domain from hAGO2

Crystal structure of MID domain from hAGO2 in complex with ATP. Determined by X-ray diffraction at 2.0 Å resolution. Released 20 Apr 2011.

Method: X-ray diffraction
Resolution: 2.0 Å
Organism: Homo sapiens
Chains: 3
Atoms: 3,429
Mol. weight: 47.5 kDa
Ligands: ATP
Released: 20 Apr 2011

Explore 3QX9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3QX9 contains 28 α-helices and 18 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	441-444	4
β-strand	448	1	1
β-strand	451-455	5	2
α-helix	464-480	17
α-helix	484	1
β-strand	485	1	1
α-helix	486	1
β-strand	491-494	4	2
α-helix	498-500	3
α-helix	501-511	11
β-strand	517-522	6	2
α-helix	528-533	6
α-helix	534-540	7
β-strand	544-548	5	2
α-helix	549-553	5
α-helix	557-571	15

Chain B: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	448	1	3
β-strand	451-455	5	4
α-helix	464-480	17
α-helix	484	1
β-strand	485	1	3
α-helix	486	1
β-strand	492-494	3	4
α-helix	498-500	3
α-helix	501-511	11
β-strand	517-522	6	4
α-helix	527-538	12
β-strand	544-548	5	4
α-helix	550-553	4
α-helix	557-571	15

Chain C: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	441-443	3
β-strand	448	1	5
β-strand	451-455	5	6
α-helix	464-480	17
α-helix	484	1
β-strand	485	1	5
α-helix	486	1
β-strand	491-494	4	6
α-helix	498-500	3
α-helix	501-511	11
β-strand	517-522	6	6
α-helix	528-533	6
α-helix	534-539	6
β-strand	544-548	5	6
α-helix	549-553	5
α-helix	557-571	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein argonaute-2	A, B, C	protein	138	Homo sapiens	Q9UKV8 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>3QX9_1 Protein argonaute-2 (chains A, B, C)
SNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQG
ADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTP
QTLSNLCLKINVKLGGVN

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	3

Primary citation

Structural analysis of 5'-mRNA-cap interactions with the human AGO2 MID domain. Frank, F., Fabian, M.R., Stepinski, J. et al. EMBO Rep (2011) 12:415-420. DOI 10.1038/embor.2011.48 · PubMed

Other PDB entries of the same protein (UniProt Q9UKV8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9BF2 1.59 Å, MID domain of Ago2 bound to UMP
4Z4D 1.6 Å, Human Argonaute2 Bound to t1-G Target RNA
3LUC 1.69 Å, Crystal structure of MID domain from hAGO2
3LUK 1.7 Å, Crystal structure of MID domain from hAGO2
7C6B 1.7 Å, Crystal structure of Ago2 MID domain in complex with 6-(3-(2-carboxyethyl)phenyl)purine…
9LSN 1.75 Å, hAGO2-MID in complex with a chemical modified uridine monophosphate
9BF0 1.78 Å, MID domain of human Argo2 bound to UTP
3LUJ 1.8 Å, Crystal structure of MID domain from hAGO2 in complex with UMP
4Z4E 1.8 Å, Human Argonaute2 Bound to t1-U Target RNA
9LMZ 1.8 Å, hAGO2-MID in complex with a chemical modified uridine monophosphate
4W5O 1.8 Å, The Crystal Structure of Human Argonaute2 Bound to a Guide and Target RNA Containing…
3LUG 1.85 Å, Crystal structure of MID domain from hAGO2 in complex with CMP

Browse structure collections

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