4B93: Vesicle-associated membrane protein 7

Complex of Vamp7 cytoplasmic domain with 2nd ankyrin repeat domain of Varp. Determined by X-ray diffraction at 2.0 Å resolution. Released 31 Oct 2012.

Method: X-ray diffraction
Resolution: 2.0 Å
Organisms: MUS MUSCULUS, HOMO SAPIENS
Chains: 2
Atoms: 2,924
Mol. weight: 51.01 kDa
Released: 31 Oct 2012

Explore 4B93 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4B93 contains 20 α-helices and 12 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	3-10	8	1
β-strand	13-19	7	1
β-strand	22-23	2	2
α-helix	25-33	9
β-strand	41-47	7	1
β-strand	50-57	8	1
β-strand	60-67	8	1
α-helix	72-90	19
α-helix	91-95	5
α-helix	97-98	2
α-helix	103-118	16
α-helix	130-134	5
β-strand	141-142	2	1
β-strand	147	1	3
β-strand	153	1	3
β-strand	158-159	2	2

Chain B: 14 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	668-678	11
α-helix	682-689	8
α-helix	747-753	7
α-helix	759-765	7
α-helix	780-787	8
α-helix	790-798	9
α-helix	813-819	7
α-helix	823-825	3
α-helix	826-831	6
β-strand	839	1	4
β-strand	845	1	4
α-helix	846-852	7
α-helix	856-864	9
α-helix	880-882	3
α-helix	884	1
α-helix	888-892	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Vesicle-associated membrane protein 7	A	protein	189	MUS MUSCULUS	P70280 (AlphaFold model)
Ankyrin repeat domain-containing protein 27	B	protein	269	HOMO SAPIENS	Q96NW4 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4B93_1 VESICLE-ASSOCIATED MEMBRANE PROTEIN 7 (chains A)
SMAILFAVVARGTTILAKHAWCGGNFLEVTEQILAKIPSENNKLTYSHGNYLFHYICQDR
IVYLCITDDDFERSRAFSFLNEVKKRFQTTYGSRAQTALPYAMNSEFSSVLAAQLKHHSE
NKSLDKVMETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTENLVDSSVTFKTTSRNLA
RAMCMKNIK

Sequence of entity 2 (B), FASTA

>4B93_2 ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 27 (chains B)
RQEETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCP
KCAPAQKRLAKVPASGLGVNVTSQDGSSPLHVAALHGRADLIPLLLKHGANAGARNADQA
VPLHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINA
SNNKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPS
CVASLDDVAETDRKEYVTVKIRKHHHHHH

Primary citation

The Binding of Varp to Vamp7 Traps Vamp7 in a Closed, Fusogenically Inactive Conformation. Schafer, I.B., Hesketh, G.G., Bright, N.A. et al. Nat Struct Mol Biol (2012) 19:1300. DOI 10.1038/NSMB.2414 · PubMed

Other PDB entries of the same protein (UniProt P70280 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2VX8 2.2 Å, Vamp7 longin domain Hrb peptide complex
4AFI 2.8 Å, Complex between Vamp7 longin domain and fragment of delta-adaptin from AP3

Browse structure collections

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