4BET: Phosphocholine transferase ankx

Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein (inactive H229A mutant) in complex with cytidine-diphosphate-choline. Determined by X-ray diffraction at 2.55 Å resolution. Released 24 Apr 2013.

Method
X-ray diffraction
Resolution
2.55 Å
Organism
LEGIONELLA PNEUMOPHILA
Chains
2
Atoms
7,853
Mol. weight
118.21 kDa
Ligands
CDC
Released
24 Apr 2013

Explore 4BET in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4BET contains 74 α-helices and 22 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 37 helices, 11 β-strands

ElementResiduesLengthSheet
α-helix11-166
α-helix19-257
α-helix29-313
α-helix39-446
α-helix48-6518
α-helix69-702
α-helix74-8512
α-helix89-924
α-helix99-1002
β-strand106-11051
α-helix111-1133
β-strand11412
α-helix116-1238
α-helix126-1305
β-strand134-13741
β-strand138-14033
β-strand145-14733
α-helix157-1593
α-helix160-17415
β-strand179-18461
α-helix190-20415
α-helix211-22717
α-helix235-2362
α-helix237-2437
α-helix245-2506
α-helix253-2564
β-strand26612
α-helix269-29123
α-helix294-2963
α-helix298-3003
α-helix305-31410
α-helix318-3247
α-helix333-34412
α-helix345-3473
α-helix352-3598
α-helix363-3697
α-helix375-3784
β-strand38214
α-helix3831
β-strand38415
β-strand38615
β-strand39114
α-helix395-4028
α-helix405-4139
α-helix428-4358
α-helix438-44912
α-helix454-4585
α-helix468-4736
Chain B: 37 helices, 11 β-strands
ElementResiduesLengthSheet
α-helix11-166
α-helix19-257
α-helix29-313
α-helix39-446
α-helix48-6518
α-helix69-702
α-helix74-8512
α-helix89-924
α-helix98-1003
β-strand106-11056
α-helix111-1133
β-strand11417
α-helix116-1238
α-helix126-1305
β-strand134-13746
β-strand138-14038
β-strand145-14738
α-helix157-1593
α-helix160-17415
β-strand179-18466
α-helix190-20516
α-helix211-22717
α-helix235-2362
α-helix237-2437
α-helix245-2506
α-helix253-2564
β-strand26617
α-helix269-29123
α-helix294-2963
α-helix298-3003
α-helix305-31410
α-helix318-3247
α-helix333-34412
α-helix345-3473
α-helix352-3598
α-helix363-3697
α-helix375-3784
β-strand38219
α-helix3831
β-strand384110
β-strand386110
β-strand39119
α-helix395-4028
α-helix405-4139
α-helix428-4358
α-helix438-44912
α-helix454-4585
α-helix468-4736

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Phosphocholine transferase ankxA, Bprotein512LEGIONELLA PNEUMOPHILAQ5ZXN6 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>4BET_1 PHOSPHOCHOLINE TRANSFERASE ANKX (chains A, B)
MSYYHHHHHHLESTSLYKKAGLENLYFQGVKIMPNLPGLYFLQAYPSEEIWRLFVDGRFW
SKENGWRGYESREPGCLNAALESLCSIALQVEKSGEEFELSVDLIKRIHKKCGKKVEELQ
EKNPGELRTDEPVSFGIPAGRASIKGIEEFLSLVFLTEGGAEFGPGKAGPFGPRFDKNYF
KNLNPEQIPDLAKQIYFDMCKYGHSNTNHFYLAVMKNVDVYLEKITQSYNKEIKTAETLD
EKLKIIVKHIRMYEVLAPFRDANGRTFVNNLLNIPLMQQGLPPATFYEPNVFDLYSAEEL
VVVVKEAIFNTVEIIEQSKRKTPITLYGYHSSLEEQTKFRDMLDSPSYEKIKHMDFSDLN
PEKLHLKTQKCLSSLNEQYPLHRGAIYLSDPGEIKLLLSNRNESQINQQIEQGAPPIYVG
KTPAHLAVISGNMAMLDELIAKKADLSLQDYDGKTALHYAAECGNMQIMGKILKVVLSQE
DAIKVLNIKDNHGKTAFHYAAEFGTPELISAL

Ligands and cofactors

IDNameFormulaCopies
CDC[2-cytidylate-O'-phosphonyloxyl]-ethyl-trimethyl-ammoniumC14 H26 N4 O11 P22

Water and common crystallization additives (SO4, GOL) are not listed.

Primary citation

Structure of the Legionella Effector Ankx Reveals the Mechanism of Phosphocholine Transfer by the Fic Domain. Campanacci, V., Mukherjee, S., Roy, C.R. et al. EMBO J (2013) 32:1469. DOI 10.1038/EMBOJ.2013.82 · PubMed

Other PDB entries of the same protein (UniProt Q5ZXN6 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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